Table 3

NAD+ depletion by MNNG in MMR-proficient and -deficient cells and inhibition of depletion by AG14361a

Cell line and MMR statusControl [NAD+] pmol/106 cells% Control [NAD+]
MNNGMNNG + AG14361
HCT + Chr3 MMR+1946 ± 700b65 ± 22NDc
HCT116 MMR−1651 ± 56147 ± 24ND
A2780 MMR+530 ± 12724 ± 697 ± 3
CP70 MMR−576 ± 8125 ± 991 ± 1
CP70-ch3 MMR+941 ± 17461 ± 10110 ± 12
CP70-ch2 MMR−1040 ± 39056 ± 10107 ± 7
  • a MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; MMR, mismatch repair.

  • b Data are mean ± SD of values determined in more than or equal to three independent experiments. Cells were exposed to 25 μm MNNG (HCT 116, HCT + chr3, CP70-ch2, and CP70-ch3) or 50 μm MNNG (A2780, CP70), ±400 nm AG14361 for 20 min before estimation of NAD+ content as described in “Materials and Methods.”

  • c ND, not determined.