Table 1

NV1066 induces apoptosis in uninfected and infected cells

Random microscopic fields of NV1066-treated cells were assessed for nuclear morphology and eGFP expression, counted, and averaged from three experiments. Cells are either apoptotic and do not express eGFP, not apoptotic and express eGFP, apoptotic and express eGFP, or not apoptotic and do not express eGFP. The last group is not represented in the table. There was a significantly higher rate of apoptosis in the infected cells compared with uninfected cells (P < 0.005, Fisher’s exact test). Untreated samples represent the spontaneous rate of apoptosis in cultured cells. Actinomycin D was used as a positive control for the induction of apoptosis.

Cell phenotype (%)
A+/GaA/G+A+/G+
Untreated0.8 ± 0.1b0.1 ± 0.10.0 ± 0.0
MOI of 0.19.6 ± 2.8b12.8 ± 2.44.4 ± 1.9
Actinomycin D (1.2 μm)18.4 ± 4.70.0 ± 0.00.0 ± 0.0
  • a A, apoptotic; G, enhanced green fluorescent protein; MOI, multiplicity of infection.

  • b P = 0.009.