Table 4.

Activities in yeast of bladder tumor p53 mutations (p53 FASAY) and p53 mutant identification by sequence analysis

Patient no.p53Pink and/or red yeast colonies (%)*
YIG-397 (RGC)YPH-p21YPH-bax
1Nonfunctional7371778317TAG (stop)
26365685175CAC (H)
31001001004110CTT (L)
46062668273CTT (L)
59696974105GAC (D)
68179805130TTC (F)
76061647234TGC (C)
81001001007236TGC (C)
98580908271AAG (K)
105554527245GTC (V)
118687825127TTC (F)
125656537-8261-2623-bp insertion
137674775175CAC (H)
1497.596974111CCG (P)
154546448285AAG (K)
161112135153ACC (T)
16Partially functional14128283CAC (H)
17235288283CAC (H)
18255308283CAC (H)
19439378283CAC (H)
  • * All the colonies were red, except those labeled in bold that appeared pink and corresponded to a partially functional p53 mutant.

  • Polymorphism 72P found at codon 72 (all the other samples at the same codon being 72R).

  • Mixture of red and pink colonies obtained from the same tumor sample; this sample was characterized by the presence of a compound heterozygous mutation showing a partially (codon 283) and a totally nonfunctional (codon 153) p53 mutants. In this tumor sample, the red colonies obtained with YPH-p21 contained only the mutant at codon 153.