Table 1.

Biodistribution of I-125-labeled anti-ED-B antibody formats in F9 tumor-bearing mice each at two selected time points

FormatL19 (scFv)2, mean (SD)
L19-SIP, mean (SD)
L19-IgG1, mean (SD)
Time points1 h24 h5 h24 h24 h96 h
Uptake in % ID/g
    Spleen2.3 (0.5)0.2 (0.0)3.2 (0.4)0.8 (0.1)1.9 (0.5)0.5 (0.4)
    Liver1.8 (0.2)0.1 (0.0)2.6 (0.3)0.5 (0.1)1.8 (0.4)0.2 (0.1)
    Kidneys27.7 (5.4)1.5 (0.8)6.9 (3.5)1.0 (0.1)1.5 (0.5)0.3 (0.2)
    Lung4.8 (0.3)0.7 (0.3)7.2 (1.9)1.4 (0.4)3.6 (1.0)0.4 (0.3)
    Bone3.2 (0.5)0.8 (0.1)5.8 (0.4)1.9 (0.6)5.5 (1.9)0.9 (1.0)
    Thyroid54.6 (39.1)214.9 (35.4)132.4 (46.8)569.8 (297.7)80.0 (78.3)152.2 (78.6)
    Muscle1.2 (0.4)0.6 (0.8)0.8 (0.1)0.3 (0.0)0.4 (0.1)0.2 (0.2)
    Tumor8.6 (2.6)3.4 (2.3)18.4 (1.8)12.6 (5.0)13.0 (5.0)3.9 (3.1)
    Blood4.7 (0.5)0.4 (0.0)8.6 (0.5)1.5 (0.1)4.9 (1.9)0.7 (0.9)
    Ovaries7.0 (2.8)1.3 (0.4)11.6 (3.3)3.1 (0.6)8.5 (3.3)1.8 (1.8)
    Uterus4.2 (0.7)0.8 (0.3)8.6 (2.6)7.3 (1.8)10.4 (5.7)2.9 (3.5)
Tumor/Tissue ratios
    T/Spleen3.7 (0.5)14.1 (8.0)5.8 (1.3)15.8 (5.1)7.5 (4.3)9.0 (4.6)
    T/Liver4.6 (0.9)25.6 (18.1)7.2 (1.5)29.7 (18.0)7.2 (1.7)19.3 (1.8)
    T/Kidneys0.3 (0.1)2.6 (2.1)3.2 (1.5)13.3 (5.9)8.6 (0.4)16.8 (9.2)
    T/Lung1.8 (0.5)5.2 (3.1)2.7 (0.9)9.6 (4.5)3.5 (0.5)8.4 (1.6)
    T/Bone2.6 (0.4)4.3 (2.2)3.2 (0.5)6.4 (0.8)2.3 (0.4)6.4 (2.9)
    T/Thyroid0.2 (0.1)0.0 (0.0)0.2 (0.1)0.0 (0.0)0.3 (0.2)0.1 (0.1)
    T/Muscle7.9 (3.2)24.5 (27.3)25.5 (7.4)48.4 (21.4)30.5 (7.8)24.9 (11.6)
    T/Blood1.8 (0.4)9.0 (5.6)2.2 (0.3)8.7 (3.9)2.7 (0.0)8.2 (3.9)
    T/Ovaries1.2 (0.1)3.0 (2.2)1.7 (0.5)4.3 (2.1)1.6 (0.9)3.3 (2.7)
    T/Uterus2.1 (0.9)4.8 (3.9)2.3 (0.6)1.8 (0.9)1.4 (0.4)1.9 (0.8)
Excretion in % ID
    Urine30.5 (6.5)67.8 (7.3)9.7 (5.7)50.2 (2.1)28.7 (13.7)67.5 (12.1)
    Faeces0.0 (0.0)3.4 (2.1)0.2 (0.3)3.2 (0.74)1.5 (1.0)6.2 (1.8)
  • NOTE: The first time point always represents maximum tumor accumulation of each respective format followed by a wash-out time point. Total excretion is measured cumulatively at indicated time points and is calculated as % injected dose. All data are presented as mean values and SD (n = 3).

    Abbreviation: ID, injected dose.