Table 2.

Biodistribution of ln-111-labeled anti-ED-B antibody formats in F9 tumor-bearing mice each at two selected time points

FormatsAP39 sc(Fv)2, mean (SD)
L19-SIP, mean (SD)
L19-IgG1, mean (SD)
Time points1 h24 h4 h24 h24 h96 h
Uptake in % ID/g
    Spleen1.9 (0.5)1.2 (0.2)4.1 (0.2)3.7 (0.5)8.1 (3.8)7.2 (0.7)
    Liver2.6 (1.3)2.3 (0.8)6.4 (0.3)7.1 (1.0)6.1 (2.4)3.3 (0.4)
    Kidneys66.9 (8.2)44.0 (3.4)22.9 (2.4)32.1 (2.9)6.0 (0.8)1.7 (0.4)
    Lung1.5 (1.6)0.8 (0.2)8.2 (0.4)3.5 (0.6)2.0 (0.7)0.7 (0.2)
    Bone3.0 (0.5)1.6 (0.2)5.5 (1.1)8.0 (1.5)10.9 (2.8)4.0 (1.3)
    Thyroid7.9 (8.6)1.7 (1.1)8.7 (3.9)3.9 (1.4)1.9 (0.7)1.1 (0.2)
    Muscle3.4 (4.5)0.4 (0.1)1.0 (0.1)1.1 (0.1)0.4 (0.1)0.3 (0.2)
    Tumor12.9 (4.8)4.3 (0.8)16.7 (5.2)14.3 (1.5)12.0 (1.9)4.9 (0.9)
    Blood5.6 (1.9)0.1 (0.0)9.4 (0.4)1.9 (0.2)2.3 (1.2)0.3 (0.2)
    Ovaries4.6 (1.5)2.5 (0.3)25.8 (6.9)11.7 (4.8)9.5 (2.2)5.4 (1.1)
    Uterus8.6 (11.8)4.3 (1.5)10.1 (4.5)18.0 (1.9)7.9 (7.4)7.8 (3.0)
Tumor/Tissue ratios
    T/Spleen7.4 (4.6)3.7 (0.2)4.1 (1.4)3.9 (0.4)1.7 (1.3)0.7 (0.1)
    T/Liver7.2 (7.0)2.0 (0.6)2.6 (0.9)2.0 (0.1)2.2 (1.1)1.5 (0.3)
    T/Kidneys0.2 (0.1)0.1 (0.0)0.8 (0.3)0.5 (0.1)2.1 (0.6)2.9 (0.2)
    T/Lung17.6 (13.3)5.8 (1.0)2.0 (0.6)4.2 (0.3)6.2 (1.7)7.4 (1.0)
    T/Bone4.5 (2.2)2.7 (0.2)3.1 (1.1)1.8 (0.4)1.2 (0.4)1.3 (0.2)
    T/Thyroid2.7 (1.7)3.2 (1.5)2.2 (1.3)4.0 (1.2)7.1 (3.1)4.6 (1.0)
    T/Muscle9.8 (7.1)9.9 (1.4)16.5 (3.6)12.8 (1.8)30.7 (12.1)19.5 (10.3)
    T/Blood2.8 (2.0)38.4 (3.8)1.8 (0.6)7.6 (0.8)6.4 (3.1)17.5 (5.5)
    T/Ovaries3.0 (1.5)1.8 (0.4)0.7 (0.4)1.3 (0.4)1.3 (0.3)0.9 (0.1)
    T/Uterus4.2 (3.2)1.1 (0.3)1.8 (0.7)0.8 (0.0)2.6 (1.8)0.7 (0.2)
Excretion in % ID
    Urine19.0 (16.8)50.3 (5.3)5.5 (1.1)20.3 (5.1)14.9 (4.5)52.7 (3.2)
    Faeces2.0 (1.9)5.1 (3.5)0.5 (0.4)4.1 (2.0)2.6 (0.7)14.6 (2.2)
  • NOTE: The first time point always represents maximum tumor accumulation of the respective format followed by a wash-out time point. Total excretion is measured cumulatively at indicated time points and is calculated as % injected dose. All data are presented as mean values and SD (n = 3).

    Abbreviation: ID, injected dose.