Table 1.

Proteins identified by two-dimensional electrophoresis and MALDI-ToF analysis

ProteinSpot*Accession no.Protein variables
Control cells
Micrometastatic cells
Calculated
Observed
MCF-7MTSV-1.7BC-K1BC-P1BC-S1
Molecular weight (kDa)Isoelectric pointMolecular weight (kDa)Isoelectric point
β-Actin1Q9633440.95.750.05.5+§++++
HSP602P1080961.05.560.05.5+++++
β-Tubulin3P0743748.85.550.05.2+++++
CK194P0872744.05.040.05.0++§
CK85P0578753.65.555.05.6++
CK8 (279 amino acids)6P0578730.85.050.05.5++
CK187P0578347.35.245.05.6++
CK18 (424 amino acids)8P0578347.35.345.05.6++
Vimentin9A2507453.65.050.04.9+++
Vimentin variant10P0867053.55.050.05.0+++
  • * Spots correspond to those marked in Fig. 1.

  • Accession numbers and calculated molecular weights derived from SwissProt/TrEMBL database.

  • Calculation based on migration of standard and known proteins.

  • § Presence (+) or absence (−) of corresponding proteins in cell lines listed.