Table 3.

Effect of AG14361 on camptothecin-induced DNA strand breaks after 16-hour exposure

Camptothecin (nmol/L)DNA single-strand breaks (relative elution)
Fold increase in strand breaks
Camptothecin aloneCamptothecin + 0.4 μmol/L AG14361
30.021 ± 0.020.08 ± 0.052.4 ± 0.7
100.12 ± 0.050.15 ± 0.041.6 ± 0.5
150.17 ± 0.060.23 ± 0.021.6 ± 0.4
300.20 ± 0.040.26 ± 0.04*1.3 ± 0.09
400.32 ± 0.060.37 ± 0.021.2 ± 0.17
1000.32 ± 0.080.35 ± 0.041.2 ± 0.14
  • NOTE: K562 cells were exposed to camptothecin in the presence or absence of 0.4 μmol/L AG14361 for 16 hours before determination of DNA single-strand breaks by alkaline elution. Relative elution was calculated by comparison with DMSO-treated or 0.4 μmol/L AG14361 alone control as appropriate. Mean ± SE for three independent experiments. Fold increase was calculated from mean ± SE relative elution values for individual experiments.

  • * P < 0.005, paired t test compared with camptothecin alone.