Specific primers for HOX genes
| Gene | Primers | Annealing temperature (°C) | Size (bp) | Intron | ||
|---|---|---|---|---|---|---|
| Sense | Antisense | |||||
| HOXA1 | ATGAACTCCTTCCTGGAATA | CGTACTCTCCAACTTTCC | 48 | 655 | + | |
| HOXA2 | ACAGCGAAGGGAAATGTAAAAGC | GGGCCCCAGAGACGCTAA | 54 | 102 | − | |
| HOXA3 | TGCAAAAAGCGACCTACTACGA | CGTCGGCGCCCAAAG | 51 | 126 | − | |
| HOXA4 | TGCATGCGAGCCACGTCCT | TTGACCTGGCGCTCAGACAA | 52 | 391 | + | |
| HOXA5 | TGCGCAAGCTGCACATAAGTCATG | TTGAAGTGGAACTCCTTCTCCAGC | 56 | 112 | + | |
| HOXA6 | GATGCAGCGCATGAACTCCTGCG | TGGGCTGCGTGGAATTGATGAGC | 58 | 250 | + | |
| HOXA7 | CAAAATGCCGAGCCGACTT | TAGCCGGACGCAAAGGG | 58 | 146 | − | |
| HOXA9 | CAGCCAACTGGCTTCATGCG | CACTCGTCTTTTGCTCGGTC | 53 | 229 | − | |
| HOXA10 | AGAGCAGCAAAGCCTCGCCGGAGAAG | GGACGCTGCGGCTAATCTCTAGGCG | 62 | 198 | + | |
| HOXA11 | ACCCGCAAAAAGCGCTGC | GAGCATGCAGGACAGTTG | 50 | 114 | − | |
| HOXA13 | GGGAGAAAGAAGCGCGTG | CGTCGTGGCTGATATCCG | 53 | 114 | − | |
| HOXB1 | CCTTCTTAGAGTACCCACTCTG | GCATCTCCAGCTGCCTCCTT | 52 | 826 | + | |
| HOXB2 | TCCTCCTTTCGAGCAAACCTTCC | AGTGGAATTCCTTCTCCAGTTCC | 52 | 353 | + | |
| HOXB3 | AGTACAAGAAGGACCAGAAGGC | TGGAGTGTAAGGCGTTCATG | 50 | 120 | − | |
| HOXB4 | GTGCAAAGAGCCCGTCGTCTACC | CGTGTCAGGTAGCGGTTGTAGTG | 56 | 161 | + | |
| HOXB5 | GTTCCACTTCAACCGCTACC | TGTCCTTCTTCCACTTCATGC | 52 | 122 | − | |
| HOXB6 | GGCGAGGCCGCCAGACATAC | ACTCGGCCTGTTTTTCTTCC | 52 | 236 | − | |
| HOXB7 | AGAGTAACTTCCGGATCTA | TCTGCTTCAGCCCTGTCTT | 48 | 274 | + | |
| HOXB8 | AGCCTCCTTGTGCAATTG | GTAACAATTGCCCACAGC | 48 | 300 | − | |
| HOXB9 | GAGCAGGGCAAAGAGTAA | CTTTCTCCTGACACCTAG | 48 | 250 | − | |
| HOXB13 | CTGGAACAGCCAGATGTGTT | TTGGCGAGAACCTTCTTCTC | 50 | 300 | + | |
| HOXC4 | GAAGGAGAAGGATCGAGATCG | GACCTGACTTTGGTGTTGGG | 53 | 124 | − | |
| HOXC5 | TGGATGACCAAACTGCACATGAGC | CAAGTTGTTGGCGATCTCTATGCG | 54 | 149 | + | |
| HOXC6 | CACCTTAGGACATAACACACAGACC | CACTTCATCCGGCGGTTCTGGAACC | 58 | 317 | + | |
| HOXC8 | GCAACTCCCTTGAAGTTTCGT | GGGGGAAGTCCAAAGGTAATA | 51 | 172 | − | |
| HOXC9 | ACGAGGAAGAAGCGCTGCCCC | GAGAACCCGGGCCACCTCATA | 60 | 133 | − | |
| HOXC10 | CTACCGCCTGGAACAACCTGTTGG | ATGGTCTTGCTAATCTCCAGGCG | 54 | 662 | + | |
| HOXC11 | GTGTATATCAACAAAGAGAAGCGG | GATAGAGGGTCCAGGAGGTTCCGG | 54 | 322 | − | |
| HOXC12 | TAATCTCCTGAATCCCGGGTTT | TGGGTAGGACAGCGAAGGC | 52 | 124 | − | |
| HOXC13 | TCCCTAGCTCGCTGCCTCT | GGTTCACGTTGTGCGACAGG | 53 | 478 | − | |
| HOXD1 | CGACCCCCATCCCTATCTAGAC | TGGAACTCGGAAGCCAACTAAA | 52 | 115 | − | |
| HOXD3 | CATCAGCAAGCAGATCTTCC | AGCGGTTGAAGTGGAATTCC | 50 | 187 | + | |
| HOXD4 | TGGATGAAGAAGGTGCACG | ACTTCATCCTCCGGTTCTGG | 50 | 220 | + | |
| HOXD8 | GGATACGATAACTTACAGAGAC | TAGAGTTTGGAAGCGACTGT | 48 | 219 | + | |
| HOXD9 | GATGAGCAAGGAGAAATGCC | AGTCGCTGGAGAGTTTCTGG | 52 | 140 | − | |
| HOXD10 | TACTCCAACCAGCAATTGGC | CTCGGATTCGATTCTCTCGG | 52 | 233 | − | |
| HOXD11 | CTTCGACCAGTTCTACGAG | CAGACGGTCTCTGTTCAGT | 50 | 457 | + | |
| HOXD12 | AGCAGGCTAAGTTCTATGCG | CAATCTGCTGCTTCGTGTAG | 50 | 380 | + | |
| HOXD13 | AGAATGGAGAACGAGTATGCC | CGGTTCTGAAACCAAATGG | 48 | 114 | − | |