Table 2

Correlation between colonoscopic findings and tissue and fecal DNA MSP assay results

Endoscopic findingsCase IDGenes and DNA sources
CDKN2A, tissue/fecessMGMT, tissue/fecesMLH1, tissue/feces
Normal colonoscopy (no polyps)1n−/U−/U−/U
2n−/U−/U−/U
3n−/U−/U−/U
4n−/U−/U−/U
5n−/U−/U−/U
6n−/U−/U−/U
7n−/U−/U−/U
8n−/U−/na−/U
9n−/U−/U−/U
10n−/U−/U−/U
11n−/U−/U−/U
12n−/U−/U−/U
13n−/U−/U−/M
14n−/M−/U−/U
15n−/U−/M−/U
16n−/U−/M−/U
17n−/U−/M−/U
18n−/M−/M−/U
19n−/M−/M−/M
20n−/na−/na−/na
21n−/na−/na−/na
22n−/na−/na−/na
23n−/na−/na−/na
24n−/na−/na−/na
25n−/na−/na−/na
Hyperplastic polyps1hna/nana/Una/U
2hU/naU/UU/U
3hU/UU/UU/U
4hU/UU/UU/U
5hU/naU/UU/U
6hU/UU/UU/U
7hU/MU/UU/U
8hU/UM/MU/U
9hU/UM/MU/U
10hM/UM/MU/U
Adenomas1aU/naU/naU/na
2aU/UU/UU/U
3aU/UU/UU/U
4aU/UU/UU/U
5aU/UU/UU/U
6aU/UU/UU/U
7aU/naU/MU/U
8aU/UU/MU/U
9aM/UU/UU/U
10aM/UU/UU/U
11aU/UM/UU/U
12ana/UM/UM/U
13aM/UM/UU/U
14aM/UM/UU/U
15aM/UM/UU/U
16aM/MU/UU/U
17aM/MU/UU/U
18aU/UM/MU/U
19aU/UM/MM/U
20aU/MM/MU/U
21aM/UM/MU/U
22aM/UM/MU/U
23aM/UM/MU/U
24aM/MM/MU/U
25aM/MM/MU/U
26aM/MM/MU/U
27aM/MM/MU/U
28aM/MM/MU/U
29aM/MM/MM/U
  • NOTE. MSP assay results from tissue samples and fecal DNA are shown respectively for study subjects with endoscopically detected polyps. Subjects were placed into the group with hyperplastic polyps if they only had hyperplastic polyps and were placed into the group with adenomas if any of the polyps they had detected on endoscopy included an adenoma. If an individual had more than one polyp and any of these polyps had a methylated allele, the polyp DNA was scored as positive. Subjects with normal colonoscopic findings have single methylation results obtained from analysis of DNA from the fecal material.

    Abbreviations: U, unmethylated; M, methylated; CRC, colorectal cancer; na, no PCR product generated.