Table 3

Cytotoxicity of SS1P and SS1P (R490A)

ImmunotoxinIC50 (ng/mL)
A431/K5A1847
SS1P0.67 ± 0.01 (0.74 ± 0.07)*3.85 ± 0.39 (4.24 ± 2.45)*
SS1P (R490A)0.48 ± 0.01 (0.46 ± 0.06)*1.19 ± 0.18 (2.38 ± 0.61)*
  • NOTE. Cytotoxicity assays were done by measuring incorporation of [3H]leucine in cells after 20 hours of treatment with indicated concentrations of immunotoxins. IC50 is the concentration that causes 50% inhibition of protein synthesis. Mean values of three experiments ± SD are shown.

  • * Cytotoxic effects were tested in WST-8 assay and expressed as a relative viability (%untreated control), mean ± SD.