Table 1.

Results of initial library screening and synthesis of 24 sublibrary pools with two defined sequences

Pool nameP1-N (H, G, S, N)*P2 (N, S)P3 (Q, N, P, S)P4 (W, N)P5 (N, F, D, W)P6-C (I, W)Sequence (N → C)
T1HNMix of Q, N, P, SMix of W, NMix of N, F, D, WMix of I, WH N XXXX
T2GNG N XXXX
T3SNS N XXXX
T4NNN N XXXX
T5HSH S XXXX
T6GSG S XXXX
T7SSS S XXXX
T8NSN S XXXX
T17Mix of H, G, S, NMix of N, SMix of Q, N, P, SMix of W, NNIXXXX N I
T18FIXXXX F I
T19DIXXXX D I
T20WIXXXX W I
T21NWXXXX N W
T22FWXXXX F W
T23DWXXXX D W
T24WWXXXX W W
  • NOTE: The most effective candidate amino acids at different positions are shown. H, G, S, and N were selected for nh2-terminal position 1 (P1-N); N and S for position 2 (P2); Q, N, P, and S for position 3 (P3); W and N for position 4 (P4); N, F, D, and W for position 5 (P5); and I and W for COOH-terminal position 6 (P6-C). Twenty-four sublibrary pools were synthesized by combinations of the selected candidate amino acids (named as T1 to T24). For example, positions 1 and 2 were fixed with one of the candidate amino acids and the rest of the four positions were synthesized with a mixture of candidate amino acids for the T1 sublibrary pool.

  • * Candidate amino acids for each position from PS-SPCL library screening.

  • Positions in which a mixture of candidate amino acids was used for the synthesis.

  • X, positions synthesized with mixtures of amino acids mentioned in the above footnote.