Table 2.

Synthesis of eight hexapeptides with defined sequences

Peptide nameP1-N (G, S)*P2 (N)P3 (Q, N)P4 (W)P5 (F, W)P6-C (I)Sequence (N → C)
Anti-Flt1GNQWFIGNQWFI
Anti-Flt2SNQWFISNQWFI
Anti-Flt3GNNWFIGNNWFI
Anti-Flt4SNNWFISNNWFI
Anti-Flt5GNQWWIGNQWWI
Anti-Flt6SNQWWISNQWWI
Anti-Flt7GNNWWIGNNWWI
Anti-Flt8SNNWWISNNWWI
  • NOTE: Based on the results obtained from the reiterative screening of the original library and subpeptide library pools (data not shown), the most important amino acids at the second, fourth, and sixth positions were found to be N, W, and I, respectively, and the sequence of amino acids at the first (G and S), third (Q and N), and fifth (F and W) positions still remain to be further determined. By the combination of the candidate amino acids, eight hexapeptides with defined sequences were synthesized and named as anti-Flt1 to anti-Flt8. The most effective hexapeptide for the inhibition of binding of coated VEGF was anti-Flt1 peptide (GNQWFI, Fig. 2).

  • * Selected amino acid(s) for each position from the screening of the 24 subpeptide library pools.