Table 5.

Establishment of T-cell lines from postvaccination PBMC of a prostate cancer patient, using autologousdendritic cells pulsed with PSA or MUC-1 peptide, shows reactivity to both PSA and MUC-1 epitopes

T-cell lineAPC onlyAPC + carcinoembryonic antigen peptideAPC + MUC-1 peptideAPC + PSA peptide
T-3-PSA<15.6<15.6<15.6314.5
T-3-MUC-1<15.6<15.6673.4<15.6
  • NOTE: T-3-PSA and T-3-MUC-1 were established by stimulating T cells isolated from patient 3 with autologous dendritic cells pulsed with PSA peptide or MUC-1 peptide for three in vitro stimulation. The effector-to-APC ratio was 10:1. Peptides were used at a concentration of 20 μg/mL. Carcinoembryonic antigen peptide was used as a negative control. Culture supernatants (24 hours) were collected and screened for the secretion of IFN-γ. Results are expressed in pg/mL IFN-γ.