Table 4.

Cox univariate and multivariate analysis for overall survival in 635 lymph node–negative patients

FactorNo. patients (%)Univariate analysis
Multivariate analysis*
HR (95% confidence interval)PHR (95% confidence interval)P
Age (y)
    ≤4086 (14)11
    41-55224 (35)0.75 (0.51-1.10)0.77 (0.52-1.14)
    56-70200 (31)0.73 (0.49-1.09)0.64 (0.33-1.22)
    >70125 (20)0.88 (0.57-1.35)0.390.80 (0.41-1.59)0.36
Menopausal status
    Premenopausal265 (42)11
    Postmenopausal370 (58)1.00 (0.76-1.29)0.971.10 (0.65-1.88)0.71
Pathologic tumor size (cm)
    ≤2260 (41)11
    >2375 (59)1.14 (0.88-1.49)0.321.04 (0.79-1.37)0.78
Grade
    Poor331 (52)11
    Unknown195 (31)0.95 (0.71-1.27)1.01 (0.75-1.36)
    Moderate and good109 (17)0.58 (0.39-0.87)0.0170.61 (0.41-0.92)0.035
ER-α mRNA level
    Continuous635 (100)0.94 (0.86-1.02)0.141.06 (0.94-1.20)0.31
PR mRNA level
    Continuous635 (100)0.91 (0.86-0.96)0.0020.89 (0.83-0.97)0.004
Additions to the base model
CCNE1 mRNA level
    Continuous635 (100)1.24 (1.15-1.35)<0.0011.27 (1.15-1.41)<0.001
CCNE1 mRNA level
    ≤median318 (50)11
    >median317 (50)2.03 (1.54-2.66)<0.0011.95 (1.43-2.65)<0.001
CCNE1 mRNA level
    Low (≤0.03)251 (40)11
    High (>0.03)384 (60)2.55 (1.87-3.49)<0.0012.45 (1.74-3.44)<0.001
CCNE1 mRNA level in stroma rich
    Low in stroma rich156 (36)11
    High in stroma rich277 (64)3.28 (2.16-4.97)<0.0013.33 (2.13-5.21)<0.001
CCNE2 mRNA level
    Continuous635 (100)1.45 (1.24-1.70)<0.0011.38 (1.17-1.63)<0.001
CCNE2 mRNA level
    ≤median318 (50)11
    >median317 (50)1.86 (1.42-2.44)<0.0011.76 (1.34-2.32)<0.001
CCNE2 mRNA level
    Low (≤1.16)464 (73)11
    High (>1.16)171 (27)1.90 (1.45-2.48)<0.0011.75 (1.33-2.30)<0.001
CCNE2 mRNA level in stroma poor
    Low in stroma poor144 (71)11
    High in stroma poor58 (29)2.62 (1.67-4.12)<0.0012.36 (1.45-3.84)<0.001
CCNE1 + CCNE2
    CCNE1 continuous635 (100)1.24 (1.15-1.35)<0.0011.23 (1.10-1.36)<0.001
    CCNE2 continuous635 (100)1.45 (1.24-1.70)<0.0011.26 (1.06-1.50)0.007
  • * Cyclins were separately introduced to the base multivariate model that included the factors age, menopausal status, tumor size, grade, and ER-α and PR mRNA levels as log-transformed continuous variables.

  • Dichotomized in high and low levels by median level (0.04; range, 0.003-2.12 for CCNE1 and 0.70; range, 0.07-9.40 for CCNE2).

  • Dichotomized in high and low levels by cut points (0.03 for CCNE1 and 1.16 for CCNE2).