Table 2.

Immunocytochemical analysis on the expression patterns of some known metastasis-related proteins in CPGL-B-transfected and empty vector–transfected SMMC7721 cells

Antibody (dilutions)/sourcespcDNA3.1/His-V5pcDNA3.1/His-V5/CPGL-B
CXCR1 (1:25)/m
CXCR2 (1:25)/m
CXCR4 (1:25)/m
CXCR5 (1:25)/m
CXCR6 (1:25)/m
MMP2 (1:25)/r
MMP9 (1:50)/g++
ANG1 (1:100)/g+ + ++ + +
ANG2 (1:100)/g+ + + ++ + + +
OPN (1:100)/g+ + ++ + +
Integrin β3 (1:50)/g+ ++
Integrin β5 (1:50)/r++ +
Integrin αvβ3 (1:10)/m+
HIF1α (1:25)/r
nm23 (1:10)/r++
CXCR3 (1:25)/m*+ + ++
MMP11 (1:50)/g*+ + ++
CD44s (1:25)/m*+ + + ++
  • NOTE: “−” and “+” indicate absence and presence of expression level, respectively. The number of “+” represents expression levels. Antibody sources are the following: mouse (m), rabbit (r), and goat (g).

  • * Metastasis-related proteins remarkably down-regulated in CPGL-B-transfected SMMC7721 cells.