Table 4.

HR-HPV–negative TP53 mutated oropharyngeal SCC

CaseTP53/p53
Homozygous deletion
9p21 FISHcyclin D1 IHCEGFR
p15INK4b
p14ARF/p16INK4a
TF53 mutationIHCex1ex2ex1βex1αex2IHCFISHMutation
18Exon 4, A1a39Pro/GCA>CCA80%NHDHDHDHDHD30%IntermediateDisomywt
19Exon 5, Lys132Arg/AAG>AGG50%NHDHDNNDisomyHighDisomywt
20Exon 5, Ala161Asp/GCC>GAC70%NNNNNHD>70%HighHigh polysomywt
21Exon 7, Arg248Trp/CGG>TGG90%NNNNNDisomyHighLow polysomyND
22Exon 6, Tyr220Cys/TAT>TGT>80%NHDHDHDHDHDHighAmplificationND
23Exon 6, His193Arg/CAT>CGT>80%NNNNNDisomyHighDisomywt
24Exon 6, Tyr205Cys/TAT>TGT>80%NNNNNDisomy60%Monosomywt
25Exon 6, Tyr220Cys/TAT>TGT50%NHDNENENEDisomy40%HighDisomyND
26Exon 6, Arg213Trp/CGA>TGG50%NNNNNHD20%HighLow polysomywt
27Exon 6, Tyr205Cys/TAT>TGT90%NHDHDHDNHDNEHDND
28Exon 7, Arg248Trp/CGG>TGG90%NNNNNHEDIntermediateDisomyND
29Exon 7, Arg248Gln/CGG>CAG90%NHDHDHDHDNv40%NEDisomywt
30Exon 7, Arg306Thr/GCA>ACA>80%NHDHDNNEDisomy>70%HighDisomyND
31Exon 7, Arg248Trp/CGG>TGG>80%NHDHDHDHDHD>70%HighAmplificationND
32Exon 7, Cys242Ser-TGC>TCC50%NHDHDHDlowHDIntermediateLow polysomyND
33Exon 7, Tyr234Cys/TAC>TGC80%NNNNNDisomy>70%High disomyGly719Glu GGA>GAA
34Exon 8, Gly266Val/GGA>GTA90%NHDHDNNDisomy>70%IntermediateLow polysomyND
35Exon 8, Pro278Leu/CCT>CTT60%NNNNNDisomyIntermediateLow polysomyND
36Exon 8, Arg282Trp/CGG>TGG>80%NHDHDNENHD>60%HighMonosomywt
37Exon 8, Arg282Gly/CGG>GGG60%NNNNNHD50%IntermediateLow polysomyND
38Exon 9, Gly325Glu/GGA>GAA70%NHDHDHDHDHDIntermediateHigh polysomyND
39Exon 10, Leu348Ser/TGG>TCG60%NENEHDHDHDHDIntermediateDisomywt
40Exon 6, Glu204Stop/GAG>TAG>80%NNHDHDHDHD20%Disomywt
41Exon 7, Glu258stop/GAA>TAANNNNNHDHighLow polysomyND
42Exon 6, Gly244stop/GAG>TAGNNNHDNDisomy30%IntermediateHigh polysomyND
43Exon 6, Arg213Stop/CGA>TGANEHDHDHDHDHD20%HighHEDwt
44Exon 6, Arg213Stop/CGA>TGANHDNNNDisomy60%HighAmplification+ low polysomyND
45Exon 8, Glu298Stop-GAG>TAGNHDHDHDHDHD30%HighDisomyND
4613317-13329 delTAGGTCTGGCCCCNHDHDHDHDHD20%NEAmplification+ low polysomyND
4714510-14524 delGACCGGCGCACAGAG50%NENNlowHDDisomy70%IntermediateAmplification+ low polysomyND
48Exon 10, del1766150%NlowlowlowlowHED30%HighDisomywt
49INTRONE 9, A17708T90%NHDHDNEHDHD>70%LowDisomywt
50Exon 7, Ser261Ser/AGT>AGCNENENENENEHED20%IntermediateLow polysomyND
45%66%62%21%90%48%
  • Abbreviations: ND, not done; +, positive; −, negative; IHC, immunohistochemistry; N, normal; HD, homozygous deletion; NE, not evaluable; HED, hemizygous deletion.