Table 1.

Biodistribution of RCR vector 4 weeks after locoregional virus infusion in immunocompetent hepatic metastasis model

Copy number of RCR vector/5 × 104 cells
MouseTumorBone marrowLiverLungKidneySmall intestineColon
ACE-GFP-12960*
ACE-GFP-22995
ACE-GFP-37890
  • NOTE: Genomic DNA, extracted from liver tumor, bone marrow, normal liver, lung, kidney, small bowel, and colon tissue of ACE-GFP-infected mice, was analyzed by quantitative real-time PCR as described in Materials and Methods. As an internal control, each sample was also amplified with mouse β-actin-specific primers.

  • * Not detectable (detection limit was 35 copies/5 × 104 cells).