Table 1.

NSCLC lines: gefitinib sensitivity, patient characteristics, EGFR mutation status, EGFR gene copy number, and EGFR and HER2 cell surface expression

Cell lineMTT
Patient characteristics
EGFR gene copy number (dual-color FISH analysis)
EGFR expression (flow cytometry)
HER2 expression (flow cytometry)
IC50 (μmol/L)GenderPYEGFR statusMean CEP 7Mean EGFREGFR gene pattern%EGFR+MFIIHC%HER2+MFIIHC
BAC
    H3580.18M0WT33HP974ND985.2ND
    H3220.25M60WT6.923.64LP100163+965.52+
Adenocarcinoma
    HCC8270.005FUnknownexon 1916.6>25.3GA8115ND293.9ND
    H32550.015F0L858R12.96>44.08GA907.3ND472.7ND
    HCC40060.02F0exon 19>9.94>12.39GA946.8NDNDNDND
    H22790.03UnknownUnknownexon 199.1215.98GA979.8ND252ND
    Calu30.3MUnknownWT4.014.61HP988.93+100373+
    H16480.38M40WT3.333.33LP8911.7ND1007.8ND
    H16501M10exon 19>3.32>8.85HP8512.8ND894.5ND
    H1253M50WT3.43.36HT100133+912.81+
    H14353F0WT5.23.8HP98143+833.21+
    H8203M0exon 195.358.37HP254ND323.3ND
    COLO6994.2FUnknownWT2.942.93HT000+000+
    H21225.9F30WT33.07HT945.12+7341+
    H4416M0WT4.34.3HP946ND844ND
    H19758F0L858R, T790M6.206.24HP967.7ND923.4ND
    A5499.6MUnknownWT2.52.5LT99143+782.40+
Squamous
    NE185MUnknownWT3.183.19HT100163+943.30+
    H17038M50WT3.064.02HP99153+652.21+
    H22610M0WT2.012.61HT100493+962.42+
    H15712.8M0WT3.13.1HT93133+621.81+
    H52013.6M0WT2.822.65HT000+000+
Large cell
    H46012.9M0WT1.031.04M96122+321.92+
  • Abbreviations: PY, pack-years; CEP, chromosome 7; HP, high polysomy; LP, low polysomy; GA, gene amplification; HT, high trisomy; LT, low trisomy; M, monosomy; IHC, immunohistochemistry; ND, not done.