Table 1.

Effects of castration and ZD6474 treatment in orthotopic AT-1 tumors

Intact day 7* (n = 6)Intact day 10* (n = 15)Castrated day 10* (n = 18)ZD6474 day 10* (n = 13)ZD6474 + castrated day 10* (n = 13)
Tumor weight (mg)15 ± 4.064 ± 5431 ± 2522 ± 2721 ± 20
Tumor cell apoptosis (%)0.44 ± 0.140.49 ± 0.281.3 ± 0.761.3 ± 0.511.9 ± 0.59§
Tumor cell proliferation (%)38 ± 7.632 ± 8.426 ± 7.635 ± 6.5§32 ± 3.8
Volume density of blood vessels (%)2.5 ± 0.293.6 ± 0.803.7 ± 0.662.4 ± 0.4§1.9 ± 0.41§
Tumor necrosis fraction (%)0.82 ± 0.932.3 ± 2.84.8 ± 4.23.7 ± 2.99.5 ± 4.1§
Tumor hypoxia fraction (%)NA56 ± 1777 ± 1673 ± 2691 ± 3.4§
  • Abbreviation: NA, not analyzed.

  • * Days after AT-1 tumor cell inoculation. Therapy with castration and/or ZD6474 was given from day 7. For details, see Materials and Methods. Values are presented as means ± SD.

  • Significantly different than in controls at day 7, P < 0.05.

  • Significantly different than in controls at day 10, P < 0.05.

  • § Significantly different than in castrated animals at day 10, P < 0.05.

  • Significantly different than in ZD6474-treated animals at day 10, P < 0.05.