Table 1.

Primer sequences used in 5′ nuclease assay (shown in the 5′→3′ direction)

Primer nameForward sequenceReverse sequenceTaqMan probe
TScctgaatcacatcgagccactgaagaatcctgagctttgggaaaattcagcttcagcgagaacccagacc
DHFRggatagttggtggcagttctgtttgcatgatccttgtcacaaatagttaagccatgaatcacccaggccatctt
GARFTgctcccttcttttaagggttcaaaccagtaactgtgactccggttgcccatgagcaagccctgga