Table 1.

Primers sequences and probes

NamePrimer sequence*
Constructs primers
    Common primer (R)5′-TGGCAGGAGCAAAGGCGCCGCTCGAGCGG-3′
    P-269bp (F)5′-CGAGCTCGGAGGTTCTGGAAGTACTTGGC-3′
    P-540bp (F)5′-CGACGCGTGTGCTGGACTCAATAAATATTG-3′
    P-1316bp (F)5′-CGACGCGTGGATCTTGGGCCAGTGGAATG-3′
    P-2218bp (F)5′-CGACGCGTGGCCTAGTTCCTAGCATAGTG-3′
Bisulfite sequencing
    1 (F)5′-ATTAGGGAGTTATAGTTTTTGG-3′
    1 (R)5′-CAACTAACCCTTTACTTACTACA-3′
    2 (R)5′-GGGGAAGATTTTTGTTTATATG-3′
    2 (F)5′-AATACCCACTCCACAACTCC-3′
    3 (F)5′-TGTGAGTTTGGTTTATTTTATGG-3′
    3 (R)5′-TACTTCCAAAACCTCAAAATCC-3′
    4 (F)5′-TTGGTGTAT(CT)GATTGGTTTTTG-3′
    4 (R)5′-CAATAACTACCATCCACTAAAATC-3′
    5 (F)5′-GGTTTTGGAAGTATTTTGTTGTG-3′
    5 (R)5′-CAACCATAAC(AG)CCTTTACTCCTA-3′
    6 (F)5′-GGGTTTAGTGGTGTTTTATGTT-3′
    6 (R)5′-ATAACATCAAAACTACTTTCTACC-3′
    7 (F)5′-GATTTTGTTATGTTTTTGTTTGG-3′
    7 (R)5′-CAAAACTCAATAAATCCTAAACA-3′
    8 (F)5′-GTAGGTTTTAGTTATTTGTTTGAA-3′
    8 (R)5′-CAATCCCAAAAACACTACATC-3′
Primer for automatic sequencing
    1 (F)5′-GTAATGAAGGTGAGTTTTATAG-3′
    1 (R)5′-CTAAATATCCTAAAAACCTATAC-3′
    2 (R)5′-ATACTACCTACTCACTTATATC-3′
    3 (F)5′-TGTGAGTTTGGTTTATTTTATGG-3′
    4 (R)5′-CAATAACTACCATCCACTAAAATC-3′
    5 (F)5′-GGTTTTGGAAGTATTTTGTTGTG-3′
    6 (F)5′-GGGTTTAGTGGTGTTTTATGTT-3′
    7 (R)5′-CAAAACTCAATAAATCCTAAACA-3′
    8 (R)5′-CAATCCCAAAAACACTACATC-3′
QRT-PCR UGT1A1
    QRT-PCR (F)5′-GACTGTCCAGGACCTATTGAGCTC-3′
    QRT-PCR (R)5′-CATTAATGTAGGCTTCAAATTCCTGG-3′
    QRT-PCR (P)5′-(FAM)ATCATGCCCAATATGGTT(MGB)-3′
  • Abbreviations: F, forward; R, reverse; P, probe; FAM, carboxyfluorescein; MGB, minor groove binder.

  • * Accession no. for UGT1A1 reference sequence: AF297093.