Primers sequences and probes
| Name | Primer sequence* | |
|---|---|---|
| Constructs primers | ||
| Common primer (R) | 5′-TGGCAGGAGCAAAGGCGCCGCTCGAGCGG-3′ | |
| P-269bp (F) | 5′-CGAGCTCGGAGGTTCTGGAAGTACTTGGC-3′ | |
| P-540bp (F) | 5′-CGACGCGTGTGCTGGACTCAATAAATATTG-3′ | |
| P-1316bp (F) | 5′-CGACGCGTGGATCTTGGGCCAGTGGAATG-3′ | |
| P-2218bp (F) | 5′-CGACGCGTGGCCTAGTTCCTAGCATAGTG-3′ | |
| Bisulfite sequencing | ||
| 1 (F) | 5′-ATTAGGGAGTTATAGTTTTTGG-3′ | |
| 1 (R) | 5′-CAACTAACCCTTTACTTACTACA-3′ | |
| 2 (R) | 5′-GGGGAAGATTTTTGTTTATATG-3′ | |
| 2 (F) | 5′-AATACCCACTCCACAACTCC-3′ | |
| 3 (F) | 5′-TGTGAGTTTGGTTTATTTTATGG-3′ | |
| 3 (R) | 5′-TACTTCCAAAACCTCAAAATCC-3′ | |
| 4 (F) | 5′-TTGGTGTAT(CT)GATTGGTTTTTG-3′ | |
| 4 (R) | 5′-CAATAACTACCATCCACTAAAATC-3′ | |
| 5 (F) | 5′-GGTTTTGGAAGTATTTTGTTGTG-3′ | |
| 5 (R) | 5′-CAACCATAAC(AG)CCTTTACTCCTA-3′ | |
| 6 (F) | 5′-GGGTTTAGTGGTGTTTTATGTT-3′ | |
| 6 (R) | 5′-ATAACATCAAAACTACTTTCTACC-3′ | |
| 7 (F) | 5′-GATTTTGTTATGTTTTTGTTTGG-3′ | |
| 7 (R) | 5′-CAAAACTCAATAAATCCTAAACA-3′ | |
| 8 (F) | 5′-GTAGGTTTTAGTTATTTGTTTGAA-3′ | |
| 8 (R) | 5′-CAATCCCAAAAACACTACATC-3′ | |
| Primer for automatic sequencing | ||
| 1 (F) | 5′-GTAATGAAGGTGAGTTTTATAG-3′ | |
| 1 (R) | 5′-CTAAATATCCTAAAAACCTATAC-3′ | |
| 2 (R) | 5′-ATACTACCTACTCACTTATATC-3′ | |
| 3 (F) | 5′-TGTGAGTTTGGTTTATTTTATGG-3′ | |
| 4 (R) | 5′-CAATAACTACCATCCACTAAAATC-3′ | |
| 5 (F) | 5′-GGTTTTGGAAGTATTTTGTTGTG-3′ | |
| 6 (F) | 5′-GGGTTTAGTGGTGTTTTATGTT-3′ | |
| 7 (R) | 5′-CAAAACTCAATAAATCCTAAACA-3′ | |
| 8 (R) | 5′-CAATCCCAAAAACACTACATC-3′ | |
| QRT-PCR UGT1A1 | ||
| QRT-PCR (F) | 5′-GACTGTCCAGGACCTATTGAGCTC-3′ | |
| QRT-PCR (R) | 5′-CATTAATGTAGGCTTCAAATTCCTGG-3′ | |
| QRT-PCR (P) | 5′-(FAM)ATCATGCCCAATATGGTT(MGB)-3′ | |
Abbreviations: F, forward; R, reverse; P, probe; FAM, carboxyfluorescein; MGB, minor groove binder.
↵* Accession no. for UGT1A1 reference sequence: AF297093.