Relative UGT1A1 expression compared with the control group for each cell line
| Colon adenocarcinoma cell lines | Control for 5-Aza-dC (PBS) | Control for TSA (DMSO) | 5-Aza-dC (fold increased) | TSA (fold increased) | 5-Aza-dC + TSA (fold increased) |
|---|---|---|---|---|---|
| HCT-116 | 1 | 1 | 19 (19) | 1 (1) | 128(128) |
| COLO-320DM | 1 | 1 | 15 (15) | 1 (1) | 75 (75) |
| HCT-15 | 309 | 244 | 2,132 (7) | 293 (1) | 4,075 (13) |
NOTE: Cells were treated as described in Materials and Methods with 5-Aza-dC alone (5 μmol/L) or in combination with trichostatin A (300 nmol/L).