Table 1.

Cell sensitivities towards 1α,25(OH)2D3, TSA, and 5-aza-dCyd

Cell lineED50CP 1α,25(OH)2D3 (nmol/L)ED50LP 1α,25(OH)2D3 (nmol/L)ED25LP TSA (nmol/L)ED50LP TSA (nmol/L)ED25LP 5-aza-dCyd (nmol/L)ED50LP 5-aza-dCyd (nmol/L)
MCF-12A20300254085130
T47-D15>1,0001045110>8,000
ZR-75-1100>1,00035100740>8,000
MCF-7100>1,0002545651,825
MDA-MB-231>100>1,0001530858,000
  • NOTE: Cellular responses were screened on two different assay formats, clonal proliferation (CP) in soft agar in 24-well plates and liquid proliferation (LP) in 96-well plates. The ED50 and ED25 were interpolated from dose-response graphs.