Expression analysis of actively translated mRNA transcripts in HCT-116 (wt-p53) and HCT-116 (null-p53) cells
| Genbank accession no. | Gene ID | Fold change | Biological function | |||
|---|---|---|---|---|---|---|
| Increased genes | ||||||
| NM_016292 | TRAP1 | +19.01 | Protein folding | |||
| NM_014474 | ASML3B | +13.51 | Carbohydrate metabolism | |||
| NM_001654 | ARAF1 | +12.79 | Cell growth and/or maintenance; intracellular signaling cascade | |||
| NM_000178 | GSS | +11.20 | Amino acid metabolism; glutathione biosynthesis; neurogenesis | |||
| NM_001225 | CASP4 | +8.83 | Apoptosis; proteolysis and peptidolysis | |||
| NM_001402 | EEF1A1 | +8.44 | Protein biosynthesis; regulation of translation | |||
| NM_000918 | P4HB | +7.86 | Electron transport | |||
| NM_004335 | BST2 | +7.28 | Cell proliferation; cell-cell signaling; development; immune response | |||
| NM_006743 | RBM3 | +6.85 | RNA processing | |||
| NM_004046 | ATP5A1 | +6.83 | ATP synthesis–coupled proton transport | |||
| NM_000546 | TP53 | +6.58 | Apoptosis; DNA recombination; DNA repair; cell cycle; transcription | |||
| NM_004317 | ASNA1 | +6.54 | Anion transport; response to arsenate | |||
| NM_004127 | GPS1 | +6.19 | c-Jun-NH2-kinase cascade; cell cycle; inactivation of mitogen-activated protein kinase | |||
| NM_000291 | PGK1 | +6.13 | Glycolysis | |||
| NM_003752 | EIF3S8 | +6.12 | Protein biosynthesis; regulation of translational initiation | |||
| NM_005500 | SAE1 | +5.85 | Protein ubiquitination | |||
| NM_000107 | DDB2 | +5.70 | Nucleotide-excision repair | |||
| AK024835 | CNN2 | +5.68 | Cytoskeleton organization; smooth muscle contraction | |||
| NM_003915 | CPNE1 | +5.62 | Lipid metabolism; vesicle-mediated transport | |||
| NM_006374 | STK25 | +5.53 | Phosphorylation; response to oxidative stress; signal transduction | |||
| NM_017916 | FLJ20643 | +5.44 | Metabolism | |||
| NM_006400 | DCTN2 | +5.35 | Cell proliferation; microtubule-based process; mitosis | |||
| NM_000474 | TWIST | +5.19 | Cell differentiation; chromosome organization and biogenesis | |||
| NM_005030 | PLK | +5.09 | Mitosis; protein amino acid phosphorylation; regulation of cell cycle | |||
| BC033103 | INPP5E | +5.04 | Proteolysis and peptidolysis | |||
| NM_032272 | MAF1 | +4.91 | Regulation of transcription, DNA-dependent | |||
| NM_001970 | EIF5A | +4.85 | Protein biosynthesis; translational initiation | |||
| NM_006201 | PCTK1 | +4.73 | Protein amino acid phosphorylation; regulation of cell cycle | |||
| NM_005654 | NR2F1 | +4.66 | Regulation of transcription, DNA-dependent; signal transduction | |||
| NM_003633 | ENC1 | +4.62 | Development; neurogenesis | |||
| NM_016016 | CGI-69 | +4.60 | Transport | |||
| NM_000967 | RPL3 | +4.58 | Protein biosynthesis | |||
| NM_016645 | NEUGRIN | +4.56 | Neuron differentiation | |||
| NM_018658 | KCNJ16 | +4.38 | Ion transport; potassium ion transport | |||
| NM_004864 | PLAB | +4.33 | Cell-cell signaling; signal transduction | |||
| NM_003624 | RANBP3 | +4.29 | Small GTPase-mediated signal transduction | |||
| NM_004559 | NSEP1 | +4.28 | Regulation of transcription; response to pest/pathogen/parasite | |||
| NM_012427 | KLK5 | +4.14 | Epidermis development; proteolysis and peptidolysis | |||
| NM_001085 | SERPINA3 | +4.12 | Inflammatory response; regulation of lipid metabolism | |||
| NM_021734 | SLC25A19 | +4.12 | Deoxynucleotide transport | |||
| NM_000175 | GPI | +4.10 | Carbohydrate metabolism; gluconeogenesis; glycolysis; hemostasis | |||
| NM_001908 | CTSB | +4.10 | Proteolysis and peptidolysis | |||
| NM_002923 | RGS2 | +4.01 | Cell cycle; G-protein signaling pathway; signal transduction | |||
| NM_000687 | AHCY | +3.95 | One-carbon compound metabolism | |||
| NM_000190 | HMBS | +3.94 | Heme biosynthesis | |||
| NM_013388 | PREB | +3.93 | Regulation of transcription, DNA dependent | |||
| NM_005410 | SEPP1 | +3.92 | Response to oxidative stress | |||
| NM_015679 | CLONE24922 | +3.87 | RNA processing | |||
| NM_021008 | DEAF1 | +3.82 | Regulation of transcription, DNA dependent | |||
| AK000822 | DKFZP564M182 | +3.75 | Protein biosynthesis | |||
| NM_000182 | HADHA | +3.74 | Fatty acid metabolism | |||
| NM_002611 | PDK2 | +3.70 | Glucose metabolism; signal transduction | |||
| NM_005802 | TP53BPL | +3.68 | Protein ubiquitination | |||
| AF016266 | TNFRSF10B | +3.59 | Caspase activation; electron transport; induction of apoptosis | |||
| NM_001536 | HRMT1L2 | +3.58 | Signal transduction; defense response; methylation | |||
| NM_133455 | LOC129080 | +3.52 | Phosphate transport | |||
| NM_001640 | APEH | +3.51 | Proteolysis and peptidolysis | |||
| NM_001416 | EIF4A1 | +3.50 | Protein biosynthesis | |||
| NM_006388 | HTATIP | +3.33 | Regulation of transcription, DNA-dependent; transcription | |||
| NM_006112 | PPIE | +3.29 | Protein folding | |||
| NM_000308 | PPGB | +3.28 | Intracellular protein transport; proteolysis and peptidolysis | |||
| NM_002046 | GAPD | +3.24 | Glucose metabolism; glycolysis | |||
| NM_001130 | AES | +3.24 | Wnt receptor signaling pathway; development; transcription | |||
| Decreased genes | ||||||
| NM_014178 | HSPC156 | −27.25 | Vesicle-mediated transport | |||
| NM_006533 | MIA | −15.34 | Cell proliferation | |||
| NM_006993 | NPM3 | −7.58 | Protein folding | |||
| NM_005554 | KRT6A | −6.62 | Ectoderm development | |||
| NM_002274 | KRT13 | −5.75 | Epidermis development | |||
| NM_003125 | SPRR1B | −4.81 | Epidermis development | |||
| NM_000117 | EMD | −4.76 | Muscle contraction; muscle development | |||
| NM_006072 | SCYA26 | −4.69 | Cell-cell signaling; immune response; signal transduction | |||
| BC014000 | LOC115509 | −4.18 | Regulation of transcription, DNA dependent | |||
| NM_024710 | FLJ23469 | −3.88 | Metabolism | |||
| NM_002906 | RDX | −3.47 | Cytoskeletal anchoring | |||