Table 4.

Expression analysis of actively translated mRNA transcripts in HCT-116 (wt-p53) and HCT-116 (null-p53) cells

Genbank accession no.Gene IDFold changeBiological function
Increased genes
    NM_016292TRAP1+19.01Protein folding
    NM_014474ASML3B+13.51Carbohydrate metabolism
    NM_001654ARAF1+12.79Cell growth and/or maintenance; intracellular signaling cascade
    NM_000178GSS+11.20Amino acid metabolism; glutathione biosynthesis; neurogenesis
    NM_001225CASP4+8.83Apoptosis; proteolysis and peptidolysis
    NM_001402EEF1A1+8.44Protein biosynthesis; regulation of translation
    NM_000918P4HB+7.86Electron transport
    NM_004335BST2+7.28Cell proliferation; cell-cell signaling; development; immune response
    NM_006743RBM3+6.85RNA processing
    NM_004046ATP5A1+6.83ATP synthesis–coupled proton transport
    NM_000546TP53+6.58Apoptosis; DNA recombination; DNA repair; cell cycle; transcription
    NM_004317ASNA1+6.54Anion transport; response to arsenate
    NM_004127GPS1+6.19c-Jun-NH2-kinase cascade; cell cycle; inactivation of mitogen-activated protein kinase
    NM_000291PGK1+6.13Glycolysis
    NM_003752EIF3S8+6.12Protein biosynthesis; regulation of translational initiation
    NM_005500SAE1+5.85Protein ubiquitination
    NM_000107DDB2+5.70Nucleotide-excision repair
    AK024835CNN2+5.68Cytoskeleton organization; smooth muscle contraction
    NM_003915CPNE1+5.62Lipid metabolism; vesicle-mediated transport
    NM_006374STK25+5.53Phosphorylation; response to oxidative stress; signal transduction
    NM_017916FLJ20643+5.44Metabolism
    NM_006400DCTN2+5.35Cell proliferation; microtubule-based process; mitosis
    NM_000474TWIST+5.19Cell differentiation; chromosome organization and biogenesis
    NM_005030PLK+5.09Mitosis; protein amino acid phosphorylation; regulation of cell cycle
    BC033103INPP5E+5.04Proteolysis and peptidolysis
    NM_032272MAF1+4.91Regulation of transcription, DNA-dependent
    NM_001970EIF5A+4.85Protein biosynthesis; translational initiation
    NM_006201PCTK1+4.73Protein amino acid phosphorylation; regulation of cell cycle
    NM_005654NR2F1+4.66Regulation of transcription, DNA-dependent; signal transduction
    NM_003633ENC1+4.62Development; neurogenesis
    NM_016016CGI-69+4.60Transport
    NM_000967RPL3+4.58Protein biosynthesis
    NM_016645NEUGRIN+4.56Neuron differentiation
    NM_018658KCNJ16+4.38Ion transport; potassium ion transport
    NM_004864PLAB+4.33Cell-cell signaling; signal transduction
    NM_003624RANBP3+4.29Small GTPase-mediated signal transduction
    NM_004559NSEP1+4.28Regulation of transcription; response to pest/pathogen/parasite
    NM_012427KLK5+4.14Epidermis development; proteolysis and peptidolysis
    NM_001085SERPINA3+4.12Inflammatory response; regulation of lipid metabolism
    NM_021734SLC25A19+4.12Deoxynucleotide transport
    NM_000175GPI+4.10Carbohydrate metabolism; gluconeogenesis; glycolysis; hemostasis
    NM_001908CTSB+4.10Proteolysis and peptidolysis
    NM_002923RGS2+4.01Cell cycle; G-protein signaling pathway; signal transduction
    NM_000687AHCY+3.95One-carbon compound metabolism
    NM_000190HMBS+3.94Heme biosynthesis
    NM_013388PREB+3.93Regulation of transcription, DNA dependent
    NM_005410SEPP1+3.92Response to oxidative stress
    NM_015679CLONE24922+3.87RNA processing
    NM_021008DEAF1+3.82Regulation of transcription, DNA dependent
    AK000822DKFZP564M182+3.75Protein biosynthesis
    NM_000182HADHA+3.74Fatty acid metabolism
    NM_002611PDK2+3.70Glucose metabolism; signal transduction
    NM_005802TP53BPL+3.68Protein ubiquitination
    AF016266TNFRSF10B+3.59Caspase activation; electron transport; induction of apoptosis
    NM_001536HRMT1L2+3.58Signal transduction; defense response; methylation
    NM_133455LOC129080+3.52Phosphate transport
    NM_001640APEH+3.51Proteolysis and peptidolysis
    NM_001416EIF4A1+3.50Protein biosynthesis
    NM_006388HTATIP+3.33Regulation of transcription, DNA-dependent; transcription
    NM_006112PPIE+3.29Protein folding
    NM_000308PPGB+3.28Intracellular protein transport; proteolysis and peptidolysis
    NM_002046GAPD+3.24Glucose metabolism; glycolysis
    NM_001130AES+3.24Wnt receptor signaling pathway; development; transcription
Decreased genes
    NM_014178HSPC156−27.25Vesicle-mediated transport
    NM_006533MIA−15.34Cell proliferation
    NM_006993NPM3−7.58Protein folding
    NM_005554KRT6A−6.62Ectoderm development
    NM_002274KRT13−5.75Epidermis development
    NM_003125SPRR1B−4.81Epidermis development
    NM_000117EMD−4.76Muscle contraction; muscle development
    NM_006072SCYA26−4.69Cell-cell signaling; immune response; signal transduction
    BC014000LOC115509−4.18Regulation of transcription, DNA dependent
    NM_024710FLJ23469−3.88Metabolism
    NM_002906RDX−3.47Cytoskeletal anchoring