Table 3.

HLA-DRB1*1502–restricted recognition of NY-ESO-137-54 peptide by HD1 CD4 T cells

EBV-B cellsHLA DRB1Number of IFNγ ELISPOTs
NY-ESO-137-54None
OY-EB-1 (HD1)*1101/*1502114 ± 11.648 ± 14.4
OY-EB-4*0901/*140124 ± 5.421 ± 4.8
OY-EB-5*150292 ± 10.528 ± 8.8
OY-EB-6*0901/*150285 ± 9.918 ± 8.6
OY-EB-7*090124 ± 3.727 ± 6.6
  • NOTE: Production of IFNγ ELISPOTs by HD1 CD4 T cells (2 × 104) stimulated twice with autologous CHP/ESO loaded-DCs against irradiated autologous and allogeneic EBV-B cells (2 × 104) pulsed with 10−5 mol/L NY-ESO-137-54. Assays were done in duplicate. Values are the means ± SD. The data are representative data from one of three experiments.