Table 3.

Primary antibodies

AntibodyCloneSourceDilutionAntigen retrieval*
Bcl-2124Dako1:20Pressure cooker
HER2CBE-356Novocastra1:40Microwave
c-Myc9E11Novocastra1:150Not used
Cyclin E13A3Novocastra1:40Pressure cooker
CK 5/6D5/16B4Dako1:100Pressure cooker
CK 17E3Novocastra1:30Pressure cooker
CK 8/185D3Novocastra1;100Pressure cooker
ER6F11/2Novocastra1:30Pressure cooker
Ki-67MIB-1Dako1:30Pressure cooker
Mcm-2BM28Transduction Laboratories1:1,000Pressure cooker
p271B4Novocastra1:20Pressure cooker
p53DO-7Dako1:100Pressure cooker
PRPgR 636Dako1:50Pressure cooker
  • * Microwave for 15 minutes in 850 W microwave; pressure cooker in 0.01 mol/L sodium citrate (pH 6) for 2 minutes.

  • Low pH retrieval buffer (Dako).