Table 3.

Human serum sample freeze/thaw stability

Freeze thaw cycleM30 (absorbance/% to control)
M65 (absorbance/% to control)
BRH84384BRH80678BRH84400BRH84358BRH84384BRH84400
10.82/100%0.58/118%0.13/87%0.84/105%0.38/112%0.14/108%
20.83/101%0.57/116%0.13/87%0.86/108%0.38/112%0.14/108%
30.81/99%0.56/114%0.13/87%0.87/109%0.38/112%0.14/108%
40.84/102%0.59/120%0.13/87%0.83/104%0.36/106%0.13/100%
50.89/109%0.61/125%0.13/87%0.80/100%0.36/106%0.13/100%
60.80/98%0.56/114%0.13/87%0.79/99%0.35/103%0.12/92%
Control0.82/100%0.49/100%0.15/100%0.80/100%0.34/100%0.13/100%
  • NOTE: Serums samples for this study were generated by thawing at room temperature and refreezing for 24 h at −70°C for each cycle. Controls were the same samples taken directly from −70°C without any freeze-thaws.