Table 1.

Immunohistochemical score for PI-9, cFLIP, and caspase-8 expression in Ewing sarcoma patients

PatientsBiopsiesResectionsMetastasesRelapse
PI-9+0 of 18*0 of 8*0 of 12*0 of 7*0 of 1*
cFLIP+18 of 188 of 812 of 126 of 61 of 1
Intensity score (0-3)3 (2-3)3 (2-3)3 (2-3)3
Ewing sarcoma positivity score (0-5)5 (3-5)5 (4-5)4 (1-5)5
Caspase-8+18 of 187 of 812 of 125 of 61 of 1
Intensity score (0-3)2 (1-3)2 (1-3)2 (1-3)2
Ewing sarcoma positivity score (0-5)4 (1-5)4 (3-5)4 (1-5)5
  • NOTE: Sections from paraffin-embedded Ewing sarcoma tumors were stained for expression of PI-9, cFLIP, and caspase-8. A total of 28 different sections from 18 patients were available for analysis. PI-9 was not detectable in any sample, whereas cFLIP and caspase-8 were detected in all patients albeit at variable intensity or percentages. Scores for staining intensity and percentage of positive tumor cells were graded from 0 to 3 and 0 to 5, respectively, as indicated in Materials and Methods. The average scores are indicated as ranges inside parentheses.

  • * Number of positive patients or sections out of the total number of patients or sections.