Summary of in vitro stimulation experiment and IFN-γ ELISPOT results
| Experiment no. | WT1DR 328 | WT1DR 423 | WT1DR 122 | WT1DR 122A1 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor HLADRB* | Response† | Donor HLADRB | Response | Donor HLADRB | Response | Donor HLADRB | Response | |||||
| 1 | 1001/1501 | +++ | 1001/1501 | +++ | 1401/15XX | — | 1401/15XX | + (heteroclitic)‡ | ||||
| 2 | 301/901 | +++ | 101/1201 | — | 1401 | + | 1401 | ++ (heteroclitic) | ||||
| 3 | 1001/1501 | ++ | 1001/1501 | — | 1401 | + | 1401 | + (heteroclitic) | ||||
| 4 | 407/1302 | NA§ | 407/1302 | NA | 104/1104 | — | 104/1104 | +++ (heteroclitic) | ||||
| 5 | 407/1302 | +++ | 407/1302 | + | 405/1302 | — | 301/1601 | NA | ||||
| 6 | 1001/1501 | — | 1001/1501 | — | 301/1601 | ++ | 405/1302 | — | ||||
| 7 | 407/1302 | — | 407/1302 | — | 1401/15XX | +++ | 15XX | +++ (heteroclitic) | ||||
| 8 | 1001/1501 | ++ | 1001/1501 | — | 15XX | + | 422/13XX | +++ (heteroclitic) | ||||
| 9 | 407/1302 | ++ | 407/1302 | + | 1401/15XX | +++ | 422/13XX | ++ (heteroclitic) | ||||
| 10 | 701/1202 | ++ | 701/1202 | — | 301/1601 | — | ||||||
| 11 | 1401/15XX | ++ | 1401/15XX | — | 1401/15XX | + | ||||||
| 12 | 407/1302 | +++ | 422/13XX | — | 1401/15XX | + | ||||||
| 13 | 422/13XX | + | 422/13XX | +++ | 15XX | +++ (heteroclitic) | ||||||
| 14 | 422/13XX | +++ | 1001/1501 | +++ | ||||||||
| 15 | 1001/1501 | +++ | ||||||||||
| No. donors | 6 | 80%∥ | 6 | 35.7%∥ | 6 | 66.6%∥ | 7 | 76.9%∥ | ||||
↵* Healthy donor CD3+/CD4+ T cells were stimulated twice with individual WT1 peptide as described in Materials and Methods. Stimulated T cells were challenged in an IFN-γ ELISPOT.
↵† ELISPOT results comparing T cell reactivity with autologous CD14+ cells pulsed with stimulating peptide versus autologous CD14+ cells pulsed with irrelevant peptide. −, negative results; +, two times background; ++, three times background; +++, four times background.
↵‡ T cells stimulated with WT1DR peptide 122A1 recognized CD14+ cells pulsed with stimulating peptide and CD14+ cells pulsed with WT1DR peptide 122.
↵§ Experiment failed.
↵∥ Percentage of experiments with a significant positive results (P ≤ 0.05).