Table 1.

Antibodies and protocols used for immunohistochemistry analyses

AntigenAntibodyDilutionRetrieval method
EGFRPharmDX (Dako)KitKit
Phosphorylated EGFR Tyr 108636-9700 (Zymed)1:1,000Microwave heat,* 1 mmol/L EDTA buffer (pH 9)
Phosphorylated Tyr4G10 (Upstate)1:100Microwave heat,* 10 mmol/L citrate buffer (pH 6)
Phosphorylated AktCR473 (Upstate)1:100Microwave heat,* 1 mmol/L EDTA buffer (pH 9)
STAT3SC8019 (Santa Cruz)1:25095°C, 40 min, target retrieval solution (pH 9.9; Dako)*
STAT5bSC1656 (Santa Cruz)1:100Microwave heat,* 10 mmol/L citrate buffer (pH 6)
Phosphorylated erk 1/2SC7383s (Santa Cruz)1:10095°C, 40 min, target retrieval solution (pH 9.9; Dako)*
p21wafSX118 (Dako)1:2595°C, 40 min, target retrieval solution (pH 9.9; Dako)*
p27SX53G8 (Dako)1:5095°C, 40 min, target retrieval solution (pH 9.9; Dako)*
  • * 3 × 5 min at 750 W.