Competitive binding assay of p53 mutant peptides to DQ7 (DQA1*0301, DQB1*0301, DQB 3.1)
| Peptide | IC50 (umol/L) |
|---|---|
| AYK (positive control) | <0.1 |
| 34P (negative control) | >10 |
| p53 mutant patient 168 | 0.58 |
| p53 wild-type patient 168 | 4.5 |
| p53 mutant patient 69 | >10 |
| p53 wild-type patient 69 | >10 |
| p53 mutant patient 226 | >10 |
| p53 wild-type patient 226 | >10 |
NOTE: p53 peptides, both mutant and wild type, were sequenced (14-mers), and IC50 was determined.