Table 1.

Information of primers

TargetAccession no.PrimersProduct length (bp)
CABYR-a/b*NM_012189Forward, 5′-GCAGTCACCACGAGTTAGTCC-3′510
NM_153768Reverse, 5′-CCTCGTTCACTTGTTGCCAT-3′
CABYR-cNM_138644Forward, 5′-CTTACTATGTATAGAGGGAATACTAC-3′430
NM_153769Reverse, 5′-GTTCACTTGTTGCCATTGCTAA-3′
β-ActinNM_001101Forward, 5′-GAGCTACGAGCTGCCTGACG-3′416
Reverse, 5′-CCTAGAAGCATTTGCGGTGG-3′
  • * We used a pair of primers to amplify a shared segment of CABYR-a and CABYR-b sequences because of the similarity of nucleotide sequences of them, which makes it difficult to distinguish them from each other and from the other three isoforms.