Table 1.

Primers used for RT-PCR

PrimerSize (bp)Reference No.
ShhSense 5′-cgcacggggacagctcggaagt-3′47727
Antisense 5′-ctgcgcggccctcgtagtgc-3′
PtchSense 5′-ggtggcacagtcaagaaca-3′498Design*
Antisense 5′-accaagagcgagaaatgg-3′
SmoSense 5′-ttaccttcagctgccacttctacg-3′32218
Antisense 5′-gccttggcaatcatcttgctcttc-3′
Gli1Sense 5′-ttcctaccagagtcccaagt-3′185Design*
Antisense 5′-ccctatgtgaagccctattt-3′
Cyclin ASense 5′-attagtttacctggacccag-3′44439
Antisense 5′-cacaaactctgctacttctg-3′
Cyclin D1Sense 5′-aggaacagaagtgcgaggagg-3′364Design*
Antisense 5′-gcacaagaggcaacgaaggt-3′
Cyclin ESense 5′-agttctcggctcgctccaggaaga-3′47539
Antisense 5′-tcttgtgtcgccatataccggtca-3′
p21Sense 5′-gtgagcgatggaacttcga-3′31239
Antisense 5′-aatctgtcatgctggtctgc-3′
p27Sense 5′-tagagctcgggccgtggct-3′87340
Antisense 5′-gtccattccatgaagtcagcgatatg-3′
N-mycSense 5′-gtcaccacattcaccatcac-3′33541
Antisense 5′-gggaaggcatcgtttgag-3′
C-mycSense 5′-gacatggtgaaccagagtttcat-3′690Design*
Antisense 5′-gttgctgatctgtctcaggactc-3′
Glyceraldehyde-3-phosphate dehydrogenaseSense 5′-acgaccactttgtcaagctc-3′22422
Antisense 5′-ggtctacatggcaactgtga-3′
  • * Design: we designed the primer sequence.