Table 2.

Axl and Gas6 mRNA and protein expression frequency in human gliomas WHO grades 2 to 4

mRNA expression (RT-PCR)*
Protein expression (IHC)
nAxl§Gas6§Axl/Gas6§nAxl§Gas6§Axl/Gas6§
Grade 43119 (61)25 (81)19 (61)5530 (55)41 (75)22 (43)
    GBM primary2413 (54)19 (79)13 (54)4624 (52)33 (72)18 (42)
    GBM secondary76 (85)6 (85)6 (86)96 (66)8 (89)4 (44)
Grade 365 (83)6 (100)5 (83)64 (67)4 (67)4 (67)
    Astrocytoma33 (100)3 (100)3 (100)42 (50)2 (50)2 (50)
    Oligodendroglioma21 (50)2 (100)1 (50)11 (100)1 (100)1 (100)
    Oligoastrocytoma11 (100)1 (100)1 (1)11 (100)1 (100)1 (100)
Grade 211511 (73)10 (67)6 (40)
    Astrocytoma176 (86)5 (71)3 (43)
    Oligodendroglioma21 (50)1 (50)1 (50)
    Oligoastrocytoma64 (66)4 (67)2 (33)
  • Abbreviations: RT-PCR, reverse transcription-PCR; IHC, immunohistochemistry.

  • * mRNA expression frequency was quantified semiquantitatively (no, low, moderate, or high).

  • Protein expression frequency was analyzed using the GIS by dividing into low/no (GIS 0-4), moderate (GIS 5-8), and high (GIS 9-12) values.

  • Number of cases.

  • § Number of cases with moderate to high expression levels (percentage).

  • No significant differences in expression frequencies between gliomas grading 2, 3, and 4 (Kruskal-Wallis test).