Table 1.

IC50 values of 1F6 conjugates tested against various human carcinoma cells grown in culture

Cell lineTumor typeCD70 copy numbers (×103)IC50 (nmol/L)
h1F6-mcMMAF(4)h1F6-mcMMAF(8)
786-ORCC190172 ± 9219 ± 11
Caki-1RCC14048 ± 4210 ± 8
Caki-2RCC170369 ± 3642 ± 20
DBTRG-05MGGBM7020 ± 0.3ND
U251GBM12015 ± 316
L428HD10523 ± 346 ± 4
LP-1MM3446 ± 2155 ± 34
  • NOTE: Cells were incubated with h1F6-mcMMAF or the corresponding nonbinding controls IgG-mcMMAF at the concentrations indicated. After 96 h of incubation, cell viability was determined using CellTiter-Glo luminescent cell viability reagent. CD70 copy numbers were determined by quantitative FACS analysis.

    Abbreviations: ND, not determined; GBM, glioblastoma multiforme; HD, Hodgkin disease; MM, multiple myeloma.