Table 4.

Comparison of hypermethylation detection for single genes on serum samples (HNSCC patients) and serum samples (controls)

A. Individual gene evaluation
GeneCase (n)Control (n)Sensitivity, % (95% CI)Specificity, % (95% CI)
HIC17037331.4 (20.9-43.6)92.5 (89.3-95.0)
PGP9.5522037.7 (2.1-18.5)97.5 (94.4-99.2)
CDH16232032.3 (20.9-45.3)73.1 (67.9-77.9)
CCND2472846.4 (1.3-17.5)98.2 (95.9-99.4)
TIMP35029610.0 (3.3-21.8)94.6 (91.4-96.9)
TGFBR2371348.1 (1.7-21.9)94.0 (88.6-97.4)
AIM1104110.0 (0.3-44.5)100 (93.0-100)
ESR16206.3 (0.2-30.2)100 (86.1-100)
CCNA1241040 (0-11.7)100 (97.2-100)
DCC271350 (0-10.5)100 (97.8-100)
MINT3128420 (0-10.2)100 (93.1-100)
p16391020 (0-7.4)100 (97.1-100)
RARB13850 (0-20.6)100 (96.5-100)
B. Best combination of genes for hypermethylation HNSCC detection on serum
Panel
Case (n)
Control (n)
Sensitivity, % (95% CI)
Specificity, % (95% CI)
CCND2 + TIMP3 + HIC1 + PGP9.54018265.0 (48.3-79.4)72.0 (64.9-78.4)
TIMP3 + HIC15227850.0 (35.8-64.2)84.5 (79.7-88.6)
CCND2 + HIC1 + PGP9.54218952.4 (36.4-68.0)81.0 (74.6-86.3)
CCND2 + HIC14924844.9 (30.7-59.8)87.1 (82.3-91.0)
TIMP3 + HIC1 + PGP9.54518357.8 (42.2-72.3)74.3 (67.4-80.5)
CCND2 + TIMP3 + HIC14617856.5 (41.1-71.1)73.6 (66.5-79.9)
CCND2 + TGFBR2 + TIMP3 + HIC1 + PGP9.53613072.2 (54.8-85.8)56.9 (48.0-65.6)
CDH1 + CCND2 + TIMP3 + HIC1 + PGP9.53920887.2 (72.6-95.7)42.3 (35.5-49.3)
TGFBR2 + TIMP3 + HIC14315860.5 (44.4-75.0)68.4 (60.5-75.5)
TGFBR2 + TIMP3 + HIC1 + PGP9.53813168.4 (51.4-82.5)58.8 (49.9-67.3)
TGFBR2 + HIC14414950.0 (34.6-65.4)76.5 (68.9-83.1)
CDH1 + TIMP3 + HIC14926769.4 (54.6-81.8)57.3 (51.1-63.3)
CDH1 + CCND2 + HIC1 + PGP9.53921776.9 (60.7-88.9)49.3 (42.5-56.2)
HIC17037331.4 (20.9-43.6)92.5 (89.3-95.0)
TGFBR2 + HIC1 + PGP9.53911856.4 (39.6-72.2)66.9 (57.7-75.3)
CCND2 + TGFBR2 + TIMP3 + HIC14012665.0 (48.3-79.4)58.7 (49.6-67.4)
CCND2 + TGFBR2 + HIC1 + PGP9.53711759.5 (42.1-75.3)65.0 (55.6-73.6)
CDH1 + TIMP3 + HIC1 + PGP9.54220981.0 (65.9-91.4)43.5 (36.7-50.6)
HIC1 + PGP9.55720238.6 (26.0-52.4)84.2 (78.4-88.9)