Table 2.

Limiting dilution analysis of the human colorectal cancer initiation cells

CaseNo.
Incidence
Latency (d)
No.
Incidence
Latency (d)
CD44+CD44
Patient 21001 of 5281000 of 5
1,0002 of 5231,0000 of 5
10,0001 of 582
2,000,0001 of 571
Patient 41002 of 5321000 of 5
1,0002 of 5281,0000 of 5
10,0000 of 5
2,000,0001 of 558
Patient 61001 of 5491000 of 5
1,0002 of 5341,0000 of 5
10,0000 of 5
2,000,0002 of 565

CD44+/CD133
CD44/CD133+
Patient 31,0000 of 51,0000 of 5
10,0001 of 54810,0000 of 5
100,0002 of 543100,0001 of 569

CD44+/CD133+
CD44/CD133
1,0000 of 51,0000 of 5
10,0001 of 54510,0000 of 5
100,0002 of 538100,0000 of 5
  • NOTE: Freshly isolated subpopulations of colorectal cancer cells were s.c. injected in Matrigel into female nude mice to generate tumors in mouse xenografts. The success rate is shown by tumor incidence (described as the number of tumors developed/number of injections) and latency (time in days when animals generated the first xenograft tumor).