Table 2.

Histopathologic features and molecular grade of microdissected samples

Characteristic(A)(B)OR (95% CI)P
TotalLow MG groupHigh MG group
n (%)n (%)n (%)
Sample type (n = 61)
    Benign epithelium7 (11.5)7 (20.6)0 (0)
    PDWA1 (1.6)1 (2.9)0 (0)
    ADH4 (6.6)4 (11.8)0 (0)
    LCIS*2 (3.3)1 (2.9)1 (3.7)
    DCIS47 (77.0)21 (61.8)26 (96.3)
DCIS histopathologic features
    DCIS nuclear grade (n = 47)
        Low10 (21.3)10 (47.6)0 (0)1
        Intermediate18 (38.3)11 (52.4)7 (26.9)7.6 (0.97 to >100)0.05
        High19 (40.4)0 (0)19 (73.1)226 (22.1 to >100)<0.0001
    Necrosis (n = 47)
        Absent/punctate11 (23.4)8 (38.1)3 (11.5)1
        Comedo36 (76.6)13 (61.9)23 (88.5)4.6 (0.89-31.4)0.07
    Architectural pattern (n = 47)
        Polarized15 (31.9)12 (57.1)3 (11.5)1
        Mixed24 (51.1)9 (42.9)15 (57.7)6.3 (1.2-44.6)0.02
        Nonpolarized8 (17.0)0 (0)8 (30.8)30.7 (3.7 to >100)<0.0001
    Cell polarization (n = 47)
        Present39 (83.0)21 (100)18 (69.2)1
        Absent8 (17.0)0 (0)8 (30.8)11.9 (1.7 to >100)0.001
    Calcification (n = 47)
        Absent14 (29.8)8 (38.1)6 (23.1)1
        Present§33 (70.2)13 (61.9)20 (76.9)2.0 (0.48-8.92)0.42
Whole tumor features
    Estrogen receptor (n = 45)
        Positive38 (84.4)20 (100)18 (72)1
        Negative7 (15.6)0 (0)7 (28)9.8 (1.3 to >100)0.02
    Progesterone receptor (n = 45)
        Positive35 (77.8)19 (95.0)16 (64)1
        Negative10 (22.2)1 (5.0)9 (36)10.2 (1.2 to >100)0.03
    HER2 (n = 44)
        Negative (0, +, ++)35 (79.5)19 (100)16 (64)1
        Positive (+++)9 (20.5)0 (0)9 (36)13.5 (1.9 to >100)0.006
    p53 (n = 43)
        Negative35 (81.4)19 (100)16 (66.7)1
        Positive8 (18.6)0 (0)8 (33.3)12.0 (1.7 to >100)0.01
  • NOTE: (A) Features of samples microdissected from invasive breast cancers assessed by gene expression microarray analysis. (B) Features of DCIS samples in low MG and high MG groups. Odds ratios were calculated by exact logistic regression analysis.

    Abbreviations: OR, odds ratio; 95% CI, 95% confidence interval; PDWA, proliferative disease without atypia; ADH, atypical ductal hyperplasia; LCIS, lobular carcinoma in situ.

  • * Includes one sample from a tumor with LCIS only and one sample of LCIS adjacent to DCIS that was separately sampled.

  • Represents all DCIS lesions that were laser microdissected, including two morphologic subtypes separately sampled from two cases.

  • Polarized architectural patterns include cribriform, papillary, and micropapillary; nonpolarized architectural patterns include solid and comedo.

  • § Includes coarse and fine/punctate calcification.

  • For 1 case, no HER2 result was obtained.

  • For 2 cases, no p53 result was obtained.