IC50 of TubA, CDP-TubA, 2-mercaptoethyl amide of TubA, and TubA-SS-TubA in human HT29 colorectal, human A2780 ovarian, and human H1299 non–small cell lung carcinoma cells
| Cell line | TubA (nmol/L) | CDP-TubA (nmol/L) | 2-mercaptoethyl amide of TubA (nmol/L) | TubA-SS-TubA (nmol/L) |
|---|---|---|---|---|
| HT29 | 1.3 | 4.9 | 4.4 | 2.5 |
| A2780 | 1.6 | 9.7 | 8.7 | 4.1 |
| NCI-H1299 | 2.8 | 23.7 | 19.0 | 6.6 |
NOTE: All IC50 values were acquired in a 48 h MTS assay and are expressed in tubulysin monomer equivalents.