Table 2.

mRNA changes after anti-VEGF treatment in U87 glioblastoma cell line

UnigeneSymbolDescriptionFold change (treated/control)
Genes up-regulated
    Hs.369675ANGPT1Angiopoietin 17.96
    Hs.553484ANGPT2Angiopoietin 25.34
    Hs.209153ANGPTL3Angiopoietin-like 314.25
    Hs.194654BAI1Brain-specific angiogenesis inhibitor 126.58
    Hs.632586CXCL10Chemokine (C-X-C motif) ligand 1018.47
    Hs.89714CXCL5Chemokine (C-X-C motif) ligand 57.22
    Hs.164021CXCL6Chemokine (C-X-C motif) ligand 615.22
    Hs.77367CXCL9Chemokine (C-X-C motif) ligand 937.60
    Hs.419815EGFEpidermal growth factor (β-urogastrone)10.04
    Hs.81071ECM1Extracellular matrix protein 14.28
    Hs.483635FGF1Fibroblast growth factor 1 (acidic)1.81
    Hs.284244FGF2Fibroblast growth factor 2 (basic)2.10
    Hs.1420FGFR3Fibroblast growth factor receptor 318.22
    Hs.160562IGF1Insulin-like growth factor 1 (somatomedin C)12.53
    Hs.1695MMP-12Matrix metallopeptidase 12 (macrophage elastase)77.89
    Hs.513617MMP-2Matrix metallopeptidase 2 (gelatinase A, 72-kDa type IV collagenase)2.36
    Hs.297413MMP-9Matrix metallopeptidase 9 (gelatinase B, 92-kDa type IV collagenase)11.15
    Hs.111779SPARCSecreted protein, acidic, cysteine-rich (osteonectin)5.04
    Hs.522632TIMP-1TIMP metallopeptidase inhibitor 16.08
    Hs.633514TIMP-2TIMP metallopeptidase inhibitor 22.24
Genes down-regulated
    Hs.789CXCL1Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, α)2.74
    Hs.89690CXCL3Chemokine (C-X-C motif) ligand 34.68
    Hs.57697HAS1Hyaluronan synthase 119.58
    Hs.482077ITGA2Integrin α2 (CD49B, α2 subunit of VLA-2 receptor)2.61
    Hs.375957ITGB2Integrin β2 (complement component 3 receptor 3 and 4 subunit)31.16
    Hs.270364LAMA1Laminin α154.82
    Hs.83169MMP-1Matrix metallopeptidase 1 (interstitial collagenase)25.22
  • NOTE: U87 cells were treated with 5 mg/mL bevacizumab for 72 h. Treated cells represent cells that invaded across the transwell after treatment. mRNA and cDNA were obtained as described in Materials and Methods. Template cDNA (25 ng) was loaded in each real-time PCR reaction. Five housekeeping genes were used as controls for each gene expression calculation.