Table 3.

Validation of microarray experiments using quantitative reverse transcription real-time PCR

miRNATissue group*Microarray experimentsQuantitative reverse transcription real-time PCR
Pair number (n)Fold change (disease/normal)PChange in disease tissuePair number (n)Fold change (disease/normal)PChange in disease tissueConsistency
hsa-miR-25EAC-T/EAC-N61.560.07Up253.420.01UpYes
hsa-miR-100EAC-T/EAC-N60.480.02Down250.600.005DownYes
hsa-miR-29cEAC-T/EAC-N62.610.02Up251.690.25UpYes
hsa-miR-140EAC-T/EAC-N62.050.002Up252.550.00006UpYes
hsa-miR-146aEAC-T/EAC-N62.610.04Up252.980.003UpYes
hsa-miR-146aHGD-T/HGD-N54.540.02Up2411.510.01UpYes
  • *We did not include the results of the Barrett's esophagus with low-grade dysplasia group because we could not identify any significantly differentially expressed miRNA in this group after multiple comparison adjustment.

  • P value of paired t test.

  • Consistency between the results of qRT-PCR and microarray experiments.