Table 4.

Mutational spectra of p53 in African American and Caucasian patients in relation to Pro/Pro phenotype

Case No.p53codonNucleotide changeAmino acid changeStructural element affected*Amino acid contactMutation typeExon-conserved area affectedTumors stage at diagnosis
African American patients (17 of 24; 71% of cases with p53 mutations)
p47270TTT>TGTPhe>CysS10 β sheetBuriedDisruptive8-Cons VIII C
p106152CCG>CTGPro>Leu-PENondisruptive5-Outside ConsIII C
p110153CCC>CTCPro>Leu-PENondisruptive5-Outside ConsIII C
p132196CGA>TGASTOPTruncated-Disruptive6-Cons IIIIV D
p138176TGC>TACCys>TyrL2 loopZn bindingNondisruptive5-Cons IIIII B
p146220TAT>CATTyr>His-BuriedNondisruptive6-Outside ConsIII C
p168282CGG>TGGArg>TrpH2 helixBuriedDisruptive8-Cons VIV D
p187213CGA> TGASTOPTruncated-Disruptive6-Outside ConsI A
p198196CGA>TGASTOPTruncated-Disruptive6-Outside ConsIII C
p222248CGG>CAGArg>GlnL3 loopDNA contactDisruptive7-Cons IVIII C
p251258GAA>GACGlu>AspS9 β sheetBuriedNondisruptive7-Cons IVIII C
p262273CGT>CATArg>HisS10 β sheetDNA contactNondisruptive8-Cons VII B
p276194CTT>CGTLeu>ArgL2 loopBuriedDisruptive6-Outside ConsIII C
p283273CGT>TGTArg>CysS10 β sheetDNA contactDisruptive8-Cond VIV D
p285175CGC>CACArg>HisL2 loopBuriedNondisruptive5-Cons IIIIV D
p291282CGG>TGGArg>TrpH2 helixBuriedDisruptive8-Cons VII B
p401273CGT>CTTArg>LeuS10 β sheetDNA contactDisruptive8-Cons VIII C
Caucasian patients (6 of 17; 35% of cases with p53 mutations)
P195175CGC>CACArg>HisL2 loopBuriedNondisruptive5-Cons IIIII B
P207242TGC>TACCys>TyrL3 loopZn bindingNondisruptive7-Cons IVII B
P243286GAA>AAAGlu>LysH2 helixPEDisruptive8-Cons VII B
P252282CGG>TGGArg>TrpH2 helixBuriedDisruptive8-Cons VIVD
P281245GGC>AGCGly>SerL3 loopBuriedNondisruptive7-Cons IVII B
P302236TAC>TGCTyr>CysS8 β sheetBuriedNondisruptive7-Cons IVII B
  • Abbreviations: Cons, evolutionarily conserved area; Outside cons, outside evolutionarily conserved area. Buried, <15% of DNA binding site surface area exposed. PE, partially exposed (>15 or <50% of DNA binding site surface area exposed); Disruptive, mutation that introduces an amino acid that disrupts the biding site; Nondisruptive, mutation that introduces an amino acid that does not disrupt the binding site.

  • * Structural element feature as described in Cho et al. (1994).

  • Contact features of amino acids with DNA.