Table 3.

Cytogenetically abnormal cells by biomarker stratified by pathologic stage of NSCLC expressed per microliter of peripheral blood: cases versus controls

CACs (mean ± SEM)
Controls (mean ± SEM), n = 24IA (mean ± SEM), n = 16IB (mean ± SEM), n = 8II (mean ± SEM), n = 7III (mean ± SEM), n = 10IV (mean ± SEM), n = 18Cases (mean ± SEM), n = 59MW*Trend
CAC/μL peripheral blood
UroVysion6.31 ± 1.1913.04 ± 3.3018.37 ± 5.3913.10 ± 5.8620.44 ± 7.0025.37 ± 8.5818.79 ± 3.160.010.14
LAVysion4.99 ± 1.3716.19 ± 3.4315.10 ± 3.7612.88 ± 7.5013.49 ± 3.0323.89 ± 7.8217.57 ± 2.82<0.0010.35
3p22.1 del/CEP37.04 ± 2.8143.75 ± 10.5817.28 ± 6.8727.09 ± 11.9534.94 ± 10.3571.12 ± 19.8245.52 ± 7.490.010.13
3p22.1 gain/CEP31.12 ± 0.5010.65 ± 3.116.08 ± 2.849.30 ± 5.095.56 ± 1.614.76 ± 2.137.23 ± 1.32<0.0010.09
10q22.3/CEP10 deletions5.58 ± 1.4420.72 ± 16.2525.74 ± 7.9018.15 ± 6.8720.86 ± 4.9335.98 ± 8.5225.78 ± 3.28<0.0010.12
10q22.3 gain/CEP10 3.44 ± 0.958.46 ± 1.707.08 ± 3.196.52 ± 2.3314.22 ± 7.9212.38 ± 5.0610.21 ± 2.120.010.33

NOTE: All P values are two-sided (to express number per milliliter, multiply by 1,000). Enumeration of FISH signals: 3p22.1 (green)/CEP3 (red) or 10q22.3 (green)/CEP10 (red)—normal: two signals for each probe; deletion: loss of one or both signals of 3p22.1 or 10q22.3 (green); monosomy: loss of signal for both probes (one red and one green signal); polysomy: more than two signals of each probe (three red and three green or more); gain: gain of one or both signals of 3p22.1 or 10q22.3 (green). LAV or URO probes—normal: 2 signals for each probe, loss: monosomy (one signal) of one probe; gain: polysomy (more than two signals) of one probe; abnormal: abnormality of two probes in one cell, loss or gain.

  • *P value derived from Mann-Whitney test (all cases versus controls).

  • P value for trend of abnormality across tumor stage.