Table 2.

Dye dilution proliferation assay

PatientPercentage of T cells proliferating in response to antigenic stimulus (CD4/CD8)
Tumor lysateKLH
Pre-vax1 wk3 moPre-vax1 wk3 mo
Post-vaxPost-vaxPost-vaxPost-vax
12.0/2.0
2
32.8/4.04.0/1.03.5/0.31.3/1.3/
4/1.8
5NDNDNDNDNDND
6
7—/6.0—/7.0—/4.0
8—/6.0—/4.0—/0.6—/2.0
9/2.14.0/6.23.9/1.1
10
11
123.4/1.71.1/3.4/
13
14
150.7/0.90.7/2.1/6.12.8/3.3
160.9/1.42.1/0.9
172.3/3.5/1.2
18NDNDNDNDNDND
192.3/2.7/0.91.4/—7.0/3.99.0/10.0
203.3/7.8/0.9
21—/0.30.7/1.4/
221.0/0.70.8/
231.2/—1.7/—1.7/—4.3/1.02.5/1.7
24/0.3
251.3/1.0/—8.0/2.65.0/1.4
260.4/0.40.6/0.9/0.30.7/

NOTE: Numbers represent the percentage of CD4 and CD8 T cells that proliferated in response to the antigenic stimulus (DCs pulsed with autologous tumor lysate or DCs pulsed with KLH) using the DDPA assay. “—” indicates that there was no proliferation significantly higher than that observed when unpulsed DCs were used as the stimulus. Values in bold are significantly higher than prevaccine values, indicating a response induced by the vaccine.

Abbreviation: ND, the assay was not done due to lack of cells.