Table 3.

Number of PBMCs secreting IFNγ in response to tumor and KLH

PatientTumor lysateKLH
Pre-vax1 wk3 moPre-vax1 wk3 mo
Post-vaxPost-vaxPost-vaxPost-vax
1
2
310264
47059
5NDNDNDNDNDND
64313
7
8
91091201094417
10
11
12
1390163112
14651413
154620
1642
174155
18NDNDNDNDNDND
19
20
2110136136
2254
23382042
24
2524
2628218810

NOTE: Numbers represent the number of cells per 3 × 105 PBMCs that secreted IFNγ in response to the antigenic stimulus in an ELISPOT assay. To measure tumor-specific responses, PBMCs were exposed to autologous tumor lysate–loaded DCs and cultured for 1 wk before stimulus by DCs pulsed with autologous tumor lysate (the Restimulation ELISPOT assay described in Materials and Methods). KLH responses were measured without prior in vitro stimulation. “—” indicates that the number of spots was not significantly higher than that observed when unpulsed DCs were used as the stimulus. Values in bold are significantly higher than prevaccine values, indicating a response induced by the vaccine.