Table 1.

Expression of neural stem cell–associated molecules and of transcription factors by CSC and FBS tumor lines

Cell lineMolecule*
c-MycNestinNanogS100A4S100A6Sall4Sox2
0627 CSC2 (70)n.d.3 (97)3 (98)4 (97)n.d.28 (100)
080125 CSC2 (79)n.d.2 (82)2 (92)2 (89)5 (100)7 (99)
080125 FBS2 (88)n.d.4 (99)2 (97)2 (84)8 (99)3 (99)
080418 CSC2 (67)35 (99)2 (82)2 (92)4 (89)4 (100)38 (100)
080418 FBS4 (89)15 (99)2 (99)1 (6)2 (43)3 (100)20 (100)
080325 CSC1 (42)n.d.3 (95)4 (97)2 (34)3 (99)15 (99)
080325 FBS1 (3)n.d.4 (99)1 (2)1 (1)n.d.8 (79)
071011 CSC1 (7)108 (99)2 (92)2 (61)3 (71)n.d.n.d.
071011 FBS1 (1)5 (97)2 (85)2 (77)2 (57)n.d.n.d.
070104 CSC2 (94)253 (75)4 (96)6 (97)5 (96)7 (78)35 (100)
EBV-B 18691 (2)1 (4)1 (6)1 (6)1 (3)n.d.5 (98)

Abbreviation: n.d., not done.

Note: Data are represented as MRFI that is the ratio between the mean of fluorescence intensity of cells stained with the selected mAb and that of the negative control; bold values indicate significant MRFI (≥ 2). The numbers in parenthesis represent the percentage of positive cells. The experiment has been repeated thrice; SD for each value ≥ 1 ≤ 4.

  • *The expression of the indicated molecules was evaluated by intracellular staining with specific mAbs and cytofluorimetric analysis; see Materials and Methods.