Table 2.

Expression of MHC class I and class II, and NKG2DLs molecules and their modulation by IFN-γ by GBM CSCs and FBS tumor cell lines

Cell lineIFN-γ*Molecule
MHC IMHC IIMICAMICBULBP-1ULBP-2ULBP-3ULBP-4
0627 CSCs7 (94)6 (31)4 (41)1 (6)1 (1)1 (5)1 (1)1 (2)
+8 (100)7 (52)4 (55)1 (5)1 (1)1 (7)1 (1)1 (1)
0627 FBS4 (81)4 (51)1 (2)1 (1)1 (2)1 (1)1 (1)3 (32)
+8 (100)4 (80)1 (3)1 (1)1 (3)1 (1)1 (1)3 (45)
080125 CSCs2 (97)1 (2)1 (4)1 (2)1 (1)1 (4)1 (3)2 (24)
+14 (100)1 (6)1 (4)1 (2)1 (2)2 (20)2 (25)2 (33)
080125 FBS3 (94)1 (2)3 (95)1 (2)1 (1)2 (14)1 (3)2 (45)
+6 (100)1 (8)3 (98)1 (3)1 (3)2 (14)1 (4)2 (47)
080418 CSCs1 (8)1 (2)1 (4)1 (1)1 (2)2 (10)3 (20)3 (40)
+3 (95)1 (6)1 (4)1 (2)1 (2)2 (26)3 (31)3 (55)
080418 FBS3 (96)1 (3)3 (95)1 (2)1 (3)1 (4)3 (15)1 (4)
+15 (96)1 (8)3 (98)1 (2)1 (3)1 (3)3 (45)1 (6)
080201 CSCs2 (65)1 (2)2 (27)2 (20)2 (15)2 (12)1 (3)2 (18)
+n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.
080201 FBS4 (99)1 (1)1 (6)1 (4)1 (4)2 (22)1 (3)2 (18)
+n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.
071011 CSCs6 (98)1 (1)1 (5)1 (4)1 (5)2 (2)1 (4)2 (21)
+n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.
071011 FBS26 (99)2 (76)2 (96)2 (93)2 (94)3 (95)4 (96)4 (98)
+n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.
080325 CSCs30 (99)1 (4)1 (5)2 (67)1 (3)2 (81)3 (80)2 (77)
+n.d.n.d.n.d.n.d.n.d.n.d.n.d.n.d.
070104 CSCs3 (20)2 (18)1 (2)1 (2)1 (3)1 (2)1 (2)1 (2)
+12 (98)4 (67)1 (3)1 (4)1 (4)1 (3)1 (2)1 (2)

Abbreviation: n.d., not done.

Note: Values are indicated as MRFI that is the ratio between the mean of fluorescence intensity of cells stained with the selected mAb and that of the negative control; bold values indicate significant MRFI (≥ 2). The numbers in parenthesis represent the percentage of positive cells. The experiment has been repeated thrice; SD for each value ≥ 1.2 ≤ 4.

  • *Cells were treated or not (±) for 48 h with 500 IU/mL of IFN-γ.

  • Expression of MHC molecules and NKG2DLs was evaluated by immunofluorescence and cytofluorimetric analysis.