Table 3.

Expression of APM in GBM CSC and FBS tumor cell lines

Cell lineMolecule*
HLAβ2-mHLA class IILMPTAP1TAP2
AA HC2710
0627 CSCs2 (76)4 (23)2 (49)1 (6)4 (100)2 (100)1 (6)1 (1)1 (4)
0627 FBS2 (80)2 (33)2 (61)2 (45)3 (98)2 (99)1 (3)1 (3)3 (53)
080125 CSCs1 (4)3 (94)2 (100)1 (8)6 (100)2 (98)1 (2)1 (6)1 (5)
080125 FBS2 (81)4 (63)4 (98)1 (6)6 (100)3 (86)2 (87)1 (3)2 (87)
080418 CSCs2 (78)1 (5)1 (3)2 (72)2 (98)2 (95)1 (3)1 (3)1 (3)
080418 FBS1 (6)3 (24)2 (94)1 (2)3 (100)2 (100)1 (4)1 (1)1 (4)
080201 CSCs14 (99)16 (99)7 (87)6 (71)14 (99)12 (100)3 (99)3 (98)6 (99)
080201 FBS7 (98)37 (98)53 (98)6 (78)20 (100)12 (97)5 (96)4 (98)7 (99)
071011 CSCs7 (80)26 (93)10 (43)6 (22)36 (99)15 (88)3 (45)2 (60)9 (88)
071011 FBS5 (78)7 (98)20 (98)2 (98)8 (99)3 (95)1 (5)1 (6)2 (97)
080325 CSCs10 (98)19 (99)9 (99)12 (99)24 (100)20 (100)4 (78)4 (89)7 (99)
070104 CSCs1 (2)1 (3)2 (45)2 (76)1 (4)1 (3)1 (2)1 (3)1 (3)
1869 EBV-B16 (96)19 (91)19 (98)10 (94)5 (82)6 (99)3 (89)4 (91)8 (94)

Note: Values are indicated as ratio between the mean of fluorescence intensity of cells stained with the selected mAb and that of the negative control; bold values indicate significant MRFI (≥ 2). The numbers in parenthesis represent the percentage of positive cells. The experiment has been repeated thrice; SD for each value ≥ 0.6 ≤ 4.

  • *Expression of APM molecules was evaluated by intracellular staining of permeabilized cells and cytofluorimetric analysis.

  • β-2 Microglobulin.

  • β-2 Microglobulin–free HLA-A heavy chain.