Table 6.

Suppression of T-cell proliferation by CSCs but not by their paired FBS tumor cells

PatientMitogenTumor cells% Divided cells*Division indexProliferation index
080125NoNo140.21
PHA/ConANo4412
PHA/ConACSCs270.31
PHA/ConAFBS tumor cells4612
OKT3No571.53
080418NoNo120.11
PHA/ConANo5412
PHA/ConACSCs300.41
PHA/ConAFBS tumor cells4612
OKT3No571.53

NOTE: PBMCs (1 × 107) of healthy donor were stained with 1 mmol/L CSFE and stimulated with PHA/concanavalin A in the presence or not of 5-d cultured CSCs or FBS tumor cell lines isolated from 080125 or 080418 GBM patients. After 5 d of incubation, cells were harvested and the CFSE profile was assessed by flow cytometry. The experiment was repeated twice for each time point (72 and 120 h) and data were consistent throughout the experiments with an SD of ≥ 1.4 ≤ 2.3 for each value. Statistical analysis of differences between means of the % divided cells, of the division index, or of the proliferation index was done by two-tailed t test. Data are referred to CD3+-gated cells.

Abbreviation: ConA, concanavalin A.

  • *% Divided cells, the percentage of cells of the original sample that divided.

  • Division index, the average number of divisions that a cell has undergone.

  • Proliferation index, the average number of divisions that the divided cells underwent.