Table 1.

TCR53 p:MHC ligand prevalence among renal cell carcinoma and normal kidney cells

HLA-A2HLA-A2 transfectionB3Z-TCR53 IL-2 (pg/mL)
Renal cell carcinoma cells
786-O*+63
A-498*+52
CCA-1≤12
CCA-1+191
CCA-7≤12
CCA-8≤12
CCA-8+≤12
CCA-9≤12
CCA-9+102
CCA-13≤12
CCA-17+433
CCA-23≤12
CCA-23+21
CCA-29+≤12
KT-2+20
KT-13+34
KT-15+152
KT-30+19
KT-53+21
KT-111+≤12
KT-187≤12
KT-187+≤12
KT-195≤12
KT-195+137
MZ-1257+61
MZ-2175≤12
MZ-2175+555
RCA-1770≤12
RCC-1.11+≤12
RCC-1.24+70
RCC-1.26+≤12
RCC-26+267
RCC-36+720
RCC-53+60
SKRC-12≤12
SKRC-12+61
SKRC-17+≤12
SKRC-28+≤12
SKRC-38+≤12
SKRC-44+≤12
SKRC-59+≤12
Normal kidney cells
NKC-2+≤12
NKC-3+≤12
NKC-4+≤12
NKC-6≤12
NKC-7+≤12
NKC-26+≤12
NKC-32≤12
NKC-33+19
NKC-36+≤12
NKC-37+≤12
NKC-38+≤12
NKC-39+≤12
NKC-40≤12
NKC-40+≤12
NKC-41+≤12
NKC-42+≤12
NKC-43+≤12
NKC-47≤12
NKC-49+≤12
RPTEC+≤12

NOTE: B3Z-TCR53 were cocultured with indicated cells and IL-2 values are listed (12 pg/mL was the background value of B3Z-TCR53 cells alone). Cell lines were collected through laboratory exchanges or generated locally. CCAs (provided by Gerharz et al., ref. 42), as well as 786-0, A498, MZ-1257, RCC-1.11, RCC-1.24, RCC-1.26, RCC-26, RCC-36, and RCC-53 are of known cc histology. NKC are short-term cultures of cells of tumor-free kidney cortices. HLA-A2 transfection was done using RNA. The HLA-A2 status of all cell lines and cultures was confirmed by flow cytometry.

  • *Indicates RCC with described VHL-inactivating mutations. A variant of 786-0 expressing the wild-type VHL after transfection (786-0 VHLwt8, provided by W. Kaelin, ref. 43) induced similar amounts of IL-2 with those of its VHL−/− parental line 786-0 (46 pg/mL; not shown).

  • RPTEC 2814-3 is a primary renal proximal tubular epithelial cell purchased from BioWhittaker.